Isachenko, Vladimir ORCID: 0000-0002-3674-543X, Du, Xinxin ORCID: 0000-0002-5128-7158, Isachenko, Evgenia, Todorov, Plamen ORCID: 0000-0003-3731-6116, Mallmann, Peter, Shcherbak, Olena and Rahimi, Gohar (2020). Banking of human ovarian tissue potentially contaminated by cancer cells: experimental model for study of cryo-stability of these cells. Cell Tissue Banking, 21 (1). S. 57 - 64. DORDRECHT: SPRINGER. ISSN 1573-6814

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Abstract

Auto-transplantation of cryopreserved ovarian tissue for cancer survivors comes with the primary concern of the possible existence of cancer cells in the transplanting tissue. Lineal cancer cells are presented by industry in form of separated cells (suspensions). Data about experimental models for evaluation of a cryopreservation effect on viability of compacted lineal cancer cells is currently limited. This study aims to develop a suitable experimental model for cryobiological investigations of compacted cancer cells obtained after in vitro culture of a cell suspension. Suspended lineal breast cancer cells (ZR-75-1 and MDA-MB-231) were in vitro cultured in AIM V medium for formation of monolayer. Evaluation of the cell viability was performed by healing assay, transmembrane cell migration, invasion assay and immunofluorescent test of F-actin. It was established the possibility of formation of monolayer from viable cancer cells, scarification of monolayer of these cells and formation of compacted fragments. It is described also a behaviour of compacted cells during cryopreservation (saturation by permeable cryoprotectants, thawing and removal of cryoprotectants). The described method can be used for cryobiological investigations of lineal suspended cancer cells in compacted form as a model of tissues contaminated by malignant cells and solid tumors.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Isachenko, VladimirUNSPECIFIEDorcid.org/0000-0002-3674-543XUNSPECIFIED
Du, XinxinUNSPECIFIEDorcid.org/0000-0002-5128-7158UNSPECIFIED
Isachenko, EvgeniaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Todorov, PlamenUNSPECIFIEDorcid.org/0000-0003-3731-6116UNSPECIFIED
Mallmann, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Shcherbak, OlenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rahimi, GoharUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-126907
DOI: 10.1007/s10561-019-09796-1
Journal or Publication Title: Cell Tissue Banking
Volume: 21
Number: 1
Page Range: S. 57 - 64
Date: 2020
Publisher: SPRINGER
Place of Publication: DORDRECHT
ISSN: 1573-6814
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
IN-VITRO; CRYOPRESERVATION; TRANSPLANTATION; PREGNANCY; LEUKEMIA; CORTEXMultiple languages
Cell Biology; Engineering, BiomedicalMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/12690

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