Badia, Mariana Beatriz ORCID: 0000-0001-7981-8910, Maurino, Veronica Graciela, Pavlovic, Tatiana, Arias, Cintia Lucia, Pagani, Maria Ayelen ORCID: 0000-0001-9592-3518, Andreo, Carlos Santiago, Saigo, Mariana, Drincovich, Maria Fabiana and Gerrard Wheeler, Mariel Claudia ORCID: 0000-0001-5232-5542 (2020). Loss of function of Arabidopsis NADP-malic enzyme 1 results in enhanced tolerance to aluminum stress. Plant J., 101 (3). S. 653 - 666. HOBOKEN: WILEY. ISSN 1365-313X

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Abstract

In acidic soils, aluminum (Al) toxicity is a significant limitation to crop production worldwide. Given its Al-binding capacity, malate allows internal as well as external detoxification strategies to cope with Al stress, but little is known about the metabolic processes involved in this response. Here, we analyzed the relevance of NADP-dependent malic enzyme (NADP-ME), which catalyzes the oxidative decarboxylation of malate, in Al tolerance. Plants lacking NADP-ME1 (nadp-me1) display reduced inhibition of root elongation along Al treatment compared with the wild type (wt). Moreover, wt roots exposed to Al show a drastic decrease in NADP-ME1 transcript levels. Although malate levels in seedlings and root exudates are similar in nadp-me1 and wt, a significant increase in intracellular malate is observed in roots of nadp-me1 after long exposure to Al. The nadp-me1 plants also show a lower H2O2 content in root apices treated with Al and no inhibition of root elongation when exposed to glutamate, an amino acid implicated in Al signaling. Proteomic studies showed several differentially expressed proteins involved in signal transduction, primary metabolism and protection against biotic and other abiotic stimuli and redox processes in nadp-me1, which may participate directly or indirectly in Al tolerance. The results indicate that NADP-ME1 is involved in adjusting the malate levels in the root apex, and its loss results in an increased content of this organic acid. Furthermore, the results suggest that NADP-ME1 affects signaling processes, such as the generation of reactive oxygen species and those that involve glutamate, which could lead to inhibition of root growth.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Badia, Mariana BeatrizUNSPECIFIEDorcid.org/0000-0001-7981-8910UNSPECIFIED
Maurino, Veronica GracielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pavlovic, TatianaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Arias, Cintia LuciaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pagani, Maria AyelenUNSPECIFIEDorcid.org/0000-0001-9592-3518UNSPECIFIED
Andreo, Carlos SantiagoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Saigo, MarianaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Drincovich, Maria FabianaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gerrard Wheeler, Mariel ClaudiaUNSPECIFIEDorcid.org/0000-0001-5232-5542UNSPECIFIED
URN: urn:nbn:de:hbz:38-127386
DOI: 10.1111/tpj.14571
Journal or Publication Title: Plant J.
Volume: 101
Number: 3
Page Range: S. 653 - 666
Date: 2020
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1365-313X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GENOME-WIDE IDENTIFICATION; OXIDATIVE STRESS; SEED MATURATION; ROOT-GROWTH; GENES; RESPONSES; REVEALS; EXPRESSION; GERMINATION; METABOLISMMultiple languages
Plant SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/12738

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