Weber, Stefanie, Hakobyan, Astghik, Zakaryan, Hovakim and Doerfler, Walter (2018). Intracellular African swine fever virus DNA remains unmethylated in infected Vero cells. Epigenomics, 10 (3). S. 289 - 300. LONDON: FUTURE MEDICINE LTD. ISSN 1750-192X

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Abstract

Aim: Sequence-specific CpG methylation of eukaryotic promoters is an important epigenetic signal for long-term gene silencing. We have now studied the methylation status of African swine fever virus (ASFV) DNA at various times after infection of Vero cells in culture. Methods & results: ASFV DNA was detectable throughout the infection cycle and was found unmethylated in productively infected Vero cells as documented by bisulfite sequencing of 13 viral DNA segments. Conclusion: ASFV DNA does not become de novo methylated in the course of infection in selected segments spread across the entire genome. Thus DNA methylation does not interfere with ASFV genome transcription. Lack of de novo methylation has previously been observed for free intracellular viral DNA in cells permissively infected with human adenoviruses, with human papillomaviruses and others.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Weber, StefanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hakobyan, AstghikUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zakaryan, HovakimUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doerfler, WalterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-194126
DOI: 10.2217/epi-2017-0131
Journal or Publication Title: Epigenomics
Volume: 10
Number: 3
Page Range: S. 289 - 300
Date: 2018
Publisher: FUTURE MEDICINE LTD
Place of Publication: LONDON
ISSN: 1750-192X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
EPSTEIN-BARR-VIRUS; ADENOVIRUS TYPE-2 DNA; VIRAL GENE-EXPRESSION; METHYLATION PATTERNS; NUCLEOTIDE-SEQUENCE; VACCINIA VIRUS; HAMSTER-CELLS; GENOMES; TRANSCRIPTION; REPLICATIONMultiple languages
Genetics & HeredityMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/19412

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