Universität zu Köln

Schiavinato, Alvise, Keene, Douglas R., Imhof, Thomas, Doliana, Roberto ORCID: 0000-0002-8324-7564, Sasaki, Takako and Sengle, Gerhard (2017). Fibulin-4 deposition requires EMILIN-1 in the extracellular matrix of osteoblasts. Sci Rep, 7. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

Full text not available from this repository.

Abstract

Tissue microenvironments formed by extracellular matrix networks play an important role in regulating tissue structure and function. Extracellular microfibrillar networks composed of fibrillins and their associated ligands such as LTBPs, fibulins, and EMILINs are of particular interest in this regard since they provide a specialized cellular microenvironment guiding proper morphology and functional behavior of specialized cell types. To understand how cellular microenvironments composed of intricate microfibrillar networks influence cell fate decisions in a contextual manner, more information about the spatiotemporal localization, deposition, and function of their components is required. By employing confocal immunofluorescence and electron microscopy we investigated the localization and extracellular matrix deposition of EMILIN-1 and -2 in tissues of the skeletal system such as cartilage and bone as well as in in vitro cultures of osteoblasts. We found that upon RNAi mediated depletion of EMILIN-1 in primary calvarial osteoblasts and MC3T3-E1 cells only fibulin-4 matrix deposition was lost while other fibulin family members or LTBPs remained unaffected. Immunoprecipitation and ELISAstyle binding assays confirmed a direct interaction between EMILIN-1 and fibulin-4. Our data suggest a new function for EMILIN-1 which implies the guidance of linear fibulin-4 matrix deposition and thereby fibulin-4 fiber formation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schiavinato, Alvise UNSPECIFIED UNSPECIFIED UNSPECIFIED Keene, Douglas R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Imhof, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Doliana, Roberto UNSPECIFIED orcid.org/0000-0002-8324-7564 UNSPECIFIED Sasaki, Takako UNSPECIFIED UNSPECIFIED UNSPECIFIED Sengle, Gerhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-224968
DOI:	10.1038/s41598-017-05835-7
Journal or Publication Title:	Sci Rep
Volume:	7
Date:	2017
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BETA TGF-BETA; BIOCHEMICAL-CHARACTERIZATION; EXPRESSION PATTERN; IN-VIVO; GROWTH; ELASTOGENESIS; FAMILY; GLYCOPROTEIN; MICROFIBRILS; FIBRILLIN-1 Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22496