Mehling, Lena-Maria, Piper, Thomas ORCID: 0000-0002-7462-6693, Spottke, Annika, Heidbreder, Anna, Young, Peter, Madea, Burkhard, Thevis, Mario and Hess, Cornelius (2017). GHB-O-beta-glucuronide in blood and urine is not a suitable tool for the extension of the detection window after GHB intake. Forensic Toxicol., 35 (2). S. 263 - 275. NEW YORK: SPRINGER. ISSN 1860-8973

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Abstract

Because of its small detection window, uncovering drug-facilitated crime after gamma-hydroxybutyric acid (GHB) intake remains a problem. The aim of this experiment was to determine endogenous concentrations of GHB and GHB-O-beta-glucuronide (GHB-Gluc) in plasma and urine samples and to compare them with concentrations after GHB intake in humans. Plasma and urine samples of volunteers (n = 50) who had never taken GHB during their lifetime (control group) were collected, and endogenous concentrations of GHB and GHB-Gluc were determined. In addition, plasma and urine samples of patients (n = 3) therapeutically taking sodium oxybate (GHB-sodium salt) were collected prior to and at different time points after the intake. GHB was determined via a liquid chromatography (LC)-tandem mass spectrometry system operated in multiple reaction monitoring mode. GHB-Gluc was detected by LC-quadrupole time-of-flight mass spectrometry. In plasma and urine samples of the control group (n = 50), endogenous concentrations of GHB-Gluc ranged from 0.011 to 0.067 mg/L and from 0.16 to 7.1 mg/L, respectively, while unconjugated GHB concentrations were less than 2 mg/L in both matrices. In contrast, after sodium oxybate administration, GHB concentrations increased markedly, and fell to below the commonly used cutoff value (plasma 4 mg/L and urine 10 mg/L) after 6-8 h in all patients. GHB-Gluc concentrations showed no significant time-dependent increase in plasma samples. In urine, GHB-Gluc concentrations increased after GHB intake, but were generally not higher than the endogenous concentrations of the control group. Therefore, it can be concluded that GHB-Gluc concentrations are not a suitable marker for extending the detection window after GHB intake.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Mehling, Lena-MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Piper, ThomasUNSPECIFIEDorcid.org/0000-0002-7462-6693UNSPECIFIED
Spottke, AnnikaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heidbreder, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Young, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Madea, BurkhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thevis, MarioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hess, CorneliusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-226362
DOI: 10.1007/s11419-016-0352-7
Journal or Publication Title: Forensic Toxicol.
Volume: 35
Number: 2
Page Range: S. 263 - 275
Date: 2017
Publisher: SPRINGER
Place of Publication: NEW YORK
ISSN: 1860-8973
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GAMMA-HYDROXYBUTYRIC ACID; PHARMACOKINETICS; NARCOLEPSY; HUMANS; SLEEP; METABOLITE; CATAPLEXY; ECSTASY; DRUGMultiple languages
ToxicologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/22636

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