Universität zu Köln

Isachenko, Vladimir, Sterzik, Karl, Maettner, Robert, Isachenko, Evgenia, Todorov, Plamen ORCID: 0000-0003-3731-6116, Rahimi, Gohar, Mallmann, Peter, Strehler, Erwin, Pereligin, Igor, Luis Alabart, Jose and Merzenich, Markus (2017). In Vitro Microvibration Increases Implantation Rate After Embryonic Cell Transplantation. Cell Transplant., 26 (5). S. 789 - 795. PUTNAM VALLEY: COGNIZANT COMMUNICATION CORP. ISSN 1555-3892

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Abstract

In natural conditions the oocyte and embryo are subjected to ever-changing dynamic processes. However, the routine assisted reproductive technologies today involve the use of static in vitro culture systems. The objective was to deteimine whether there is any difference in the viability of embryos after in vitro culture under static and mechanical microvibration conditions. The viability of embryonic cells (9,624 embryos) generated from 4,436 couples after in vitro culture was evaluated. For groups <= 29, 30-34, 35-39, and >= 40 years, the following rates of high-quality embryos without fragmentation (two to four blastomeres on day 2; six to eight blastomeres and compacting morula on day 3; blastocyst, expanded and hatching blastocyst on day 5) were detected (static vs. vibration, respectively): 65% versus 71%, 44% versus 69%, 67% versus 76% (for statistically significant differences between respective rates in these three groups, p <0.05), and 67% versus 66% (p> 0.1). The following baby-take-home rates were determined for groups <= 29, 30-34, 35-39, and >= 40 years (static vs. vibration, respectively): 30% versus 31% (p>0.1, increasing only on the level of tendency), 28% versus 37%, 23% versus 29%, and 9% versus 15% (differences between respective rates in these three groups with p <0.05). It was concluded that in vitro culture of embryos under microvibration (with a mimic of conditions in nature whereby oviductal fluid is mechanically agitated by the epithelial cilia) significantly increases the baby-take-home rate for patients 30 years and older.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Isachenko, Vladimir UNSPECIFIED UNSPECIFIED UNSPECIFIED Sterzik, Karl UNSPECIFIED UNSPECIFIED UNSPECIFIED Maettner, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Todorov, Plamen UNSPECIFIED orcid.org/0000-0003-3731-6116 UNSPECIFIED Rahimi, Gohar UNSPECIFIED UNSPECIFIED UNSPECIFIED Mallmann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Strehler, Erwin UNSPECIFIED UNSPECIFIED UNSPECIFIED Pereligin, Igor UNSPECIFIED UNSPECIFIED UNSPECIFIED Luis Alabart, Jose UNSPECIFIED UNSPECIFIED UNSPECIFIED Merzenich, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-246289
DOI:	10.3727/096368916X693428
Journal or Publication Title:	Cell Transplant.
Volume:	26
Number:	5
Page Range:	S. 789 - 795
Date:	2017
Publisher:	COGNIZANT COMMUNICATION CORP
Place of Publication:	PUTNAM VALLEY
ISSN:	1555-3892
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Cell & Tissue Engineering; Medicine, Research & Experimental; Transplantation Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24628