Frerichs, Anneke, Thoma, Rahere, Abdallah, Ali Taleb, Frommolt, Peter ORCID: 0000-0002-1966-8014, Werr, Wolfgang and Chandler, John William (2016). The founder-cell transcriptome in the Arabidopsis apetala1 cauliflower inflorescence meristem. BMC Genomics, 17. LONDON: BMC. ISSN 1471-2164

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Abstract

Background: Although the pattern of lateral organ formation from apical meristems establishes species-specific plant architecture, the positional information that confers cell fate to cells as they transit to the meristem flanks where they differentiate, remains largely unknown. We have combined fluorescence-activated cell sorting and RNA-seq to characterise the cell-type-specific transcriptome at the earliest developmental time-point of lateral organ formation using DORNROSCHEN-LIKE::GFP to mark founder-cell populations at the periphery of the inflorescence meristem (IM) in apetala1 cauliflower double mutants, which overproliferate IMs. Results: Within the lateral organ founder-cell population at the inflorescence meristem, floral primordium identity genes are upregulated and stem-cell identity markers are downregulated. Additional differentially expressed transcripts are involved in polarity generation and boundary formation, and in epigenetic and post-translational changes. However, only subtle transcriptional reprogramming within the global auxin network was observed. Conclusions: The transcriptional network of differentially expressed genes supports the hypothesis that lateral organ founder-cell specification involves the creation of polarity from the centre to the periphery of the IM and the establishment of a boundary from surrounding cells, consistent with bract initiation. However, contrary to the established paradigm that sites of auxin response maxima pre-pattern lateral organ initiation in the IM, auxin response might play a minor role in the earliest stages of lateral floral initiation.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Frerichs, AnnekeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thoma, RahereUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Abdallah, Ali TalebUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Frommolt, PeterUNSPECIFIEDorcid.org/0000-0002-1966-8014UNSPECIFIED
Werr, WolfgangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chandler, John WilliamUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-255948
DOI: 10.1186/s12864-016-3189-x
Journal or Publication Title: BMC Genomics
Volume: 17
Date: 2016
Publisher: BMC
Place of Publication: LONDON
ISSN: 1471-2164
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
JAGGED-LATERAL-ORGANS; SHOOT APICAL MERISTEM; GENE-EXPRESSION MAP; FLORAL-MERISTEM; AUXIN DEPLETION; DNA METHYLATION; HOMEOTIC GENE; RNA-SEQ; ARABIDOPSIS; LEAFMultiple languages
Biotechnology & Applied Microbiology; Genetics & HeredityMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/25594

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