Correia, Claudia, Koshkin, Alexey, Carido, Madalena ORCID: 0000-0001-8969-0877, Espinha, Nuno, Saric, Tomo ORCID: 0000-0001-8344-1095, Lima, Pedro A., Serra, Margarida ORCID: 0000-0001-5013-6625 and Alves, Paula M. (2016). Effective Hypothermic Storage of Human Pluripotent Stem Cell-Derived Cardiomyocytes Compatible With Global Distribution of Cells for Clinical Applications and Toxicology Testing. Stem Cells Transl. Med., 5 (5). S. 658 - 670. HOBOKEN: WILEY. ISSN 2157-6580
Full text not available from this repository.Abstract
To fully explore the potential of human pluripotent stem cell derived cardiomyocytes (hPSC-CMs), efficient methods for storage and shipment of these cells are required. Here, we evaluated the feasibility to cold store monolayers and aggregates of functional CMs obtained from different PSC lines using a fully defined clinical-compatible preservation formulation and investigated the time frame that hPSC-CMs could be subjected to hypothermic storage. We showed that two-dimensional (2D) monolayers of hPSC-CMs can be efficiently stored at 4 degrees C for 3 days without compromising cell viability. However, cell viability decreased when the cold storage interval was extended to 7 days. We demonstrated that hPSC-CMs are more resistant to prolonged hypothermic storage-induced cell injury in three-dimensional aggregates than in 2D monolayers, showing high cell recoveries (>70%) after 7 days of storage. Importantly, hPSC-CMs maintained their typical (ultra)structure, gene and protein expression profile, electrophysiological profiles, and drug responsiveness.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-278099 | ||||||||||||||||||||||||||||||||||||
DOI: | 10.5966/sctm.2015-0238 | ||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Stem Cells Transl. Med. | ||||||||||||||||||||||||||||||||||||
Volume: | 5 | ||||||||||||||||||||||||||||||||||||
Number: | 5 | ||||||||||||||||||||||||||||||||||||
Page Range: | S. 658 - 670 | ||||||||||||||||||||||||||||||||||||
Date: | 2016 | ||||||||||||||||||||||||||||||||||||
Publisher: | WILEY | ||||||||||||||||||||||||||||||||||||
Place of Publication: | HOBOKEN | ||||||||||||||||||||||||||||||||||||
ISSN: | 2157-6580 | ||||||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||||||
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Refereed: | Yes | ||||||||||||||||||||||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/27809 |
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