Correia, Claudia, Koshkin, Alexey, Carido, Madalena ORCID: 0000-0001-8969-0877, Espinha, Nuno, Saric, Tomo ORCID: 0000-0001-8344-1095, Lima, Pedro A., Serra, Margarida ORCID: 0000-0001-5013-6625 and Alves, Paula M. (2016). Effective Hypothermic Storage of Human Pluripotent Stem Cell-Derived Cardiomyocytes Compatible With Global Distribution of Cells for Clinical Applications and Toxicology Testing. Stem Cells Transl. Med., 5 (5). S. 658 - 670. HOBOKEN: WILEY. ISSN 2157-6580

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Abstract

To fully explore the potential of human pluripotent stem cell derived cardiomyocytes (hPSC-CMs), efficient methods for storage and shipment of these cells are required. Here, we evaluated the feasibility to cold store monolayers and aggregates of functional CMs obtained from different PSC lines using a fully defined clinical-compatible preservation formulation and investigated the time frame that hPSC-CMs could be subjected to hypothermic storage. We showed that two-dimensional (2D) monolayers of hPSC-CMs can be efficiently stored at 4 degrees C for 3 days without compromising cell viability. However, cell viability decreased when the cold storage interval was extended to 7 days. We demonstrated that hPSC-CMs are more resistant to prolonged hypothermic storage-induced cell injury in three-dimensional aggregates than in 2D monolayers, showing high cell recoveries (>70%) after 7 days of storage. Importantly, hPSC-CMs maintained their typical (ultra)structure, gene and protein expression profile, electrophysiological profiles, and drug responsiveness.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Correia, ClaudiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koshkin, AlexeyUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Carido, MadalenaUNSPECIFIEDorcid.org/0000-0001-8969-0877UNSPECIFIED
Espinha, NunoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Saric, TomoUNSPECIFIEDorcid.org/0000-0001-8344-1095UNSPECIFIED
Lima, Pedro A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Serra, MargaridaUNSPECIFIEDorcid.org/0000-0001-5013-6625UNSPECIFIED
Alves, Paula M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-278099
DOI: 10.5966/sctm.2015-0238
Journal or Publication Title: Stem Cells Transl. Med.
Volume: 5
Number: 5
Page Range: S. 658 - 670
Date: 2016
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 2157-6580
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
COLD PRESERVATION; CARDIAC MYOCYTES; RAT HEARTS; IN-VITRO; HEPATOCYTES; TRANSPLANTATION; DIFFERENTIATION; EXPANSION; MOUSE; INTEGRITYMultiple languages
Cell & Tissue EngineeringMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/27809

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