Burnouf, Sylvie, Groenke, Sebastian, Augustin, Hrvoje, Dols, Jacqueline, Gorsky, Marianna Karina, Werner, Jennifer, Kerr, Fiona ORCID: 0000-0003-3097-1951, Alic, Nazif ORCID: 0000-0003-0356-6600, Martinez, Pedro and Partridge, Linda ORCID: 0000-0001-9615-0094 (2016). Deletion of endogenous Tau proteins is not detrimental in Drosophila. Sci Rep, 6. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Human Tau (hTau) is a highly soluble and natively unfolded protein that binds to microtubules within neurons. Its dysfunction and aggregation into insoluble paired helical filaments is involved in the pathogenesis of Alzheimer's disease (AD), constituting, together with accumulated beta-amyloid (A beta) peptides, a hallmark of the disease. Deciphering both the loss-of-function and toxic gain-of-function of hTau proteins is crucial to further understand the mechanisms leading to neurodegeneration in AD. As the fruit fly Drosophila melanogaster expresses Tau proteins (dTau) that are homologous to hTau, we aimed to better comprehend dTau functions by generating a specific tau knock-out (KO) fly line using homologous recombination. We observed that the specific removal of endogenous dTau proteins did not lead to overt, macroscopic phenotypes in flies. Indeed, survival, climbing ability and neuronal function were unchanged in tau KO flies. In addition, we did not find any overt positive or negative effect of dTau removal on human A beta-induced toxicity. Altogether, our results indicate that the absence of dTau proteins has no major functional impact on flies, and suggests that our tau KO strain is a relevant model to further investigate the role of dTau proteins in vivo, thereby giving additional insights into hTau functions.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Burnouf, SylvieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Groenke, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Augustin, HrvojeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dols, JacquelineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gorsky, Marianna KarinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Werner, JenniferUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kerr, FionaUNSPECIFIEDorcid.org/0000-0003-3097-1951UNSPECIFIED
Alic, NazifUNSPECIFIEDorcid.org/0000-0003-0356-6600UNSPECIFIED
Martinez, PedroUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Partridge, LindaUNSPECIFIEDorcid.org/0000-0001-9615-0094UNSPECIFIED
URN: urn:nbn:de:hbz:38-281449
DOI: 10.1038/srep23102
Journal or Publication Title: Sci Rep
Volume: 6
Date: 2016
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2045-2322
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BETA TOXICITY; PHOSPHORYLATION; MODELS; PAR-1; DEGENERATION; EXPRESSION; DEFICITS; FIBER; APPMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/28144

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