Universität zu Köln

Nakayama, Yoshitaka ORCID: 0000-0001-6797-5556, Becker, Michael, Ebrahimian, Haleh, Konishi, Tomoyuki, Kawasaki, Hisashi, Kraemer, Reinhard and Martinac, Boris ORCID: 0000-0001-8422-7082 (2016). The impact of the C-terminal domain on the gating properties of MscCG from Corynebacterium glutamicum. Biochim. Biophys. Acta-Biomembr., 1858 (1). S. 130 - 139. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1879-2642

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Abstract

The mechanosensitive (MS) channel MscCG from the soil bacterium Corynebacterium glutamicum functions as a major glutamate exporter. MscCG belongs to a subfamily of the bacterial MscS-like channels, which play an important role in osmoregulation. To understand the structural and functional features of MscCG, we investigated the role of the carboxyl-terminal domain, whose relevance for the channel gating has been unknown. The chimeric channel MscS-(C-MscCG), which is a fusion protein between the carboxyl terminal domain of MscCG and the MscS channel, was examined by the patch clamp technique. We found that the chimeric channel exhibited MS channel activity in Escherichia coli spheroplasts characterized by a lower activation threshold and slow closing compared to MscS. The chimeric channel MscS-(C-MscCG) was successfully reconstituted into azolectin liposomes and exhibited gating hysteresis in a voltage-dependent manner, especially at high pipette voltages. Moreover, the channel remained open after releasing pipette pressure at membrane potentials physiologically relevant for C glutamicum. This contribution to the gating hysteresis of the C-terminal domain of MscCG confers to the channel gating properties highly suitable for release of intracellular solutes. (C) 2015 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nakayama, Yoshitaka UNSPECIFIED orcid.org/0000-0001-6797-5556 UNSPECIFIED Becker, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Ebrahimian, Haleh UNSPECIFIED UNSPECIFIED UNSPECIFIED Konishi, Tomoyuki UNSPECIFIED UNSPECIFIED UNSPECIFIED Kawasaki, Hisashi UNSPECIFIED UNSPECIFIED UNSPECIFIED Kraemer, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Martinac, Boris UNSPECIFIED orcid.org/0000-0001-8422-7082 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-292113
DOI:	10.1016/j.bbamem.2015.10.010
Journal or Publication Title:	Biochim. Biophys. Acta-Biomembr.
Volume:	1858
Number:	1
Page Range:	S. 130 - 139
Date:	2016
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1879-2642
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MECHANOSENSITIVE CHANNEL MSCS; ESCHERICHIA-COLI; ION-CHANNEL; ELECTROPHYSIOLOGICAL CHARACTERIZATION; GLUTAMATE SECRETION; MYCOLIC ACIDS; NCGL1221; MEMBRANE; RECONSTITUTION; PURIFICATION Multiple languages Biochemistry & Molecular Biology; Biophysics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/29211