Universität zu Köln

Isachenko, Vladimir ORCID: 0000-0002-3674-543X, Morgenstern, Bernd, Todorov, Plamen ORCID: 0000-0003-3731-6116, Isachenko, Evgenia, Mallmann, Peter, Hanstein, Bettina and Rahimi, Gohar (2020). Patient with ovarian insufficiency: baby born after anticancer therapy and re-transplantation of cryopreserved ovarian tissue. J. Ovarian Res., 13 (1). LONDON: BMC. ISSN 1757-2215

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Abstract

Background The second major cause of death is cancer. In fact, the effectiveness of anticancer treatments and positive long-term prognosis for young women has increased. However, the problem of post-cancer infertility plays a significant role, because chemotherapy can be gonadotoxic and lead to the functional death of ovaries. There is potential key solution to this problem: cryopreservation of ovarian tissue before cancer therapy with re-implantation after convalescence. Data regarding cryopreservation and re-transplantation of ovarian tissue from patients with ovarian insufficiency is limited. The aim of this treatment was the re-transplantation of cryopreserved ovarian tissue after anticancer therapy of patient with ovarian insufficiency (56 IU/l FSH, 8 ng/l beta-estradiol, < 1.1 ng/ml anti-Mullerian hormone, 1 primary follicle per 10mm(3)). Case presentation After the operation, four tissue fragments (10-16 x 8-13 x 1.0-1.2 mm) were cooled to 5 degrees C in the freezing medium (culture medium+ 6% ethylene glycol+ 6% dimethyl sulfoxide+ 0.15 M sucrose) for 24 h, frozen and thawed. Freezing was performed in four standard 5 ml cryo-vials with ice formation at - 9 degrees C, cooling from - 9 to - 34 degrees C at a rate of - 0.3 degrees C/min and plunging at - 34 degrees C into liquid nitrogen. After thawing in a 100 degrees C (boiling) water bath, the removal of cryoprotectants was performed in 0.5 M sucrose with 20 min. exposure in sucrose and 30 min. stepping rehydration. After thawing of one cryo-vial, part (5 mm(3)) of experimental ovarian tissue after 7 day in vitro culture was histological evaluated and two ovarian fragments (8 x 7 x 1.0 mm and 7 x 6 x 1.0 mm) were re-transplanted. The quantity of follicles after cryopreservation and in vitro culture was not increased (P > 0.1): it was found 1 primordial follicle in 5 mm(3)of tissue. Thirty seven days after the re-transplantation of ovarian tissue, the restoration of the menstrual cycle of Patient W. was noted. Three months after the transplantation, the patient became spontaneously pregnant and delivered a healthy baby girl at term. Conclusions Described protocol of conventional cryopreservation of ovarian tissue can be used for treatment of patients with ovarian insufficiency.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Isachenko, Vladimir UNSPECIFIED orcid.org/0000-0002-3674-543X UNSPECIFIED Morgenstern, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Todorov, Plamen UNSPECIFIED orcid.org/0000-0003-3731-6116 UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Mallmann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Hanstein, Bettina UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahimi, Gohar UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-318037
DOI:	10.1186/s13048-020-00713-9
Journal or Publication Title:	J. Ovarian Res.
Volume:	13
Number:	1
Date:	2020
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1757-2215
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VITRO ACTIVATION; FERTILITY PRESERVATION; FOLLICLES; AUTOTRANSPLANTATION; VITRIFICATION; PREGNANCY Multiple languages Reproductive Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31803