Wierbowski, Shayne D., Vo, Tommy V., Falter-Braun, Pascal ORCID: 0000-0003-2012-6746, Jobe, Timothy O., Kruse, Lars H., Wei, Xiaomu, Liang, Jin, Meyer, Michael J., Akturk, Nurten, Rivera-Erick, Christen A., Cordero, Nicolas A., Paramo, Mauricio I., Shayhidin, Elnur E., Bertolotti, Marta, Tippens, Nathaniel D., Akther, Kazi, Sharma, Rita, Katayose, Yuichi, Salehi-Ashtiani, Kourosh ORCID: 0000-0002-6521-5243, Hao, Tong, Ronald, Pamela C., Ecker, Joseph R., Schweitzer, Peter A., Kikuchi, Shoshi, Mizuno, Hiroshi, Hill, David E., Vidal, Marc ORCID: 0000-0003-3391-5410, Moghe, Gaurav D., McCouch, Susan R. and Yu, Haiyuan (2020). A massively parallel barcoded sequencing pipeline enables generation of the first ORFeome and interactome map for rice. Proc. Natl. Acad. Sci. U. S. A., 117 (21). S. 11836 - 11843. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop PCR-mediated Linkage of barcoded Adapters To nucleic acid Elements for sequencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for Oryza sativa covering 2,300 genes and constructing a high-quality protein-protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein-protein interactions in a wide range of organisms.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Wierbowski, Shayne D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vo, Tommy V.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Falter-Braun, PascalUNSPECIFIEDorcid.org/0000-0003-2012-6746UNSPECIFIED
Jobe, Timothy O.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kruse, Lars H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wei, XiaomuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liang, JinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meyer, Michael J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Akturk, NurtenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rivera-Erick, Christen A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cordero, Nicolas A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Paramo, Mauricio I.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Shayhidin, Elnur E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bertolotti, MartaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tippens, Nathaniel D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Akther, KaziUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sharma, RitaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Katayose, YuichiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Salehi-Ashtiani, KouroshUNSPECIFIEDorcid.org/0000-0002-6521-5243UNSPECIFIED
Hao, TongUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ronald, Pamela C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ecker, Joseph R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schweitzer, Peter A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kikuchi, ShoshiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mizuno, HiroshiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hill, David E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vidal, MarcUNSPECIFIEDorcid.org/0000-0003-3391-5410UNSPECIFIED
Moghe, Gaurav D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
McCouch, Susan R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yu, HaiyuanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-332868
DOI: 10.1073/pnas.1918068117
Journal or Publication Title: Proc. Natl. Acad. Sci. U. S. A.
Volume: 117
Number: 21
Page Range: S. 11836 - 11843
Date: 2020
Publisher: NATL ACAD SCIENCES
Place of Publication: WASHINGTON
ISSN: 0027-8424
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
NETWORK EVOLUTION; STRESS-RESPONSE; VERSION 1.1; PROTEOME; DATABASE; ANNOTATION; PLATFORM; CLONING; PROTEASOME; PROTEINSMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/33286

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