Universität zu Köln

Oldewurtel, Enno R., Kouzel, Nadzeya, Dewenter, Lena, Henseler, Katja and Maier, Berenike ORCID: 0000-0001-6971-9927 (2015). Differential interaction forces govern bacterial sorting in early biofilms. eLife, 4. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD. ISSN 2050-084X

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Abstract

Bacterial biofilms can generate micro-heterogeneity in terms of surface structures. However, little is known about the associated changes in the physics of cell-cell interaction and its impact on the architecture of biofilms. In this study, we used the type IV pilus of Neisseria gonorrhoeae to test whether variation of surface structures induces cell-sorting. We show that the rupture forces between pili are fine-tuned by post-translational modification. Bacterial sorting was dependent on pilus post-translational modification and pilus density. Active force generation was necessary for defined morphologies of mixed microcolonies. The observed morphotypes were in remarkable agreement with the differential strength of adhesion hypothesis proposing that a tug-of-war among surface structures of different cells governs cell sorting. We conclude that in early biofilms the density and rupture force of bacterial surface structures can trigger cell sorting based on similar physical principles as in developing embryos.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Oldewurtel, Enno R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kouzel, Nadzeya UNSPECIFIED UNSPECIFIED UNSPECIFIED Dewenter, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Henseler, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Maier, Berenike UNSPECIFIED orcid.org/0000-0001-6971-9927 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-392686
DOI:	10.7554/eLife.10811
Journal or Publication Title:	eLife
Volume:	4
Date:	2015
Publisher:	ELIFE SCIENCES PUBLICATIONS LTD
Place of Publication:	CAMBRIDGE
ISSN:	2050-084X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Physics > Institut für Biologische Physik
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PILIN ANTIGENIC VARIATION; CELL-CELL ADHESION; IV PILI; TWITCHING MOTILITY; NEISSERIA; DEFINE; ASSOCIATION; DYNAMICS; PATHWAY; TISSUES Multiple languages Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/39268