Isachenko, Evgenia, Isachenko, Vladimir, Mallmann, Peter, Orth, Ingrid, Peters, Doris, Schmidt, Torsten, Morgenstern, Bernd, Foth, Dolores, Hanstein, Bettina, Roethlisberger, Maria and Rahimi, Gohar (2014). Transplantation of Cryopreserved Human Ovarian Tissue: Restoration of Reproductive Function after Two Cycles of Radio - and Chemo-Therapeutic Treatments. Clin. Lab., 60 (2). S. 245 - 253. HEIDELBERG: CLIN LAB PUBL. ISSN 1433-6510

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Abstract

Background: Patient S. was born in 1983, developed a Ewing-Sarcoma and obtained low dose chemotherapy in 1996. In 2007, Patient S. received high-dose chemotherapy because lung-metastases were diagnosed. The aim of the study was to investigate the health of cryopreserved ovarian tissue and also to examine whether this ovarian tissue can restore the reproductive function of patient after two cycles of radio-and chemo-therapeutic treatments. Methods: Twenty pieces of ovarian tissue (total of similar to 200 mm(2)) were conventionally frozen with 6% (v/v) dimethyl sulfoxide, 6% (v/v) ethylene glycol and 0.15 M sucrose and kept for five years before 8 pieces were thawed and transplanted back into the patient. Two small (1 x 2 x 1 mm) pieces of this thawed tissue were cultured in a chicken embryonic chorioallantoic membrane (CAM)-system for 5 days to assess the tissue viability. Results: The ovarian tissue that was grafted re-established spontaneous menstrual bleeding within five months and serum 17-beta estradiol increased from 19 to 330 pg/mL. Ultrasound revealed a dominant follicle at the site of the transplanted tissue in the follicular phase after the menstrual bleed. Analysis of the CAM cultured tissue established that 88% of the primordial follicles had degenerated and there was limited growth of blood vessels. Conclusions: In spite of the damage caused by the cryopreservation the surviving follicles could restore ovarian function after re-transplantation.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Isachenko, EvgeniaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Isachenko, VladimirUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mallmann, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Orth, IngridUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Peters, DorisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schmidt, TorstenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Morgenstern, BerndUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Foth, DoloresUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hanstein, BettinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Roethlisberger, MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rahimi, GoharUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-449512
DOI: 10.7754/Clin.Lab.2013.130239
Journal or Publication Title: Clin. Lab.
Volume: 60
Number: 2
Page Range: S. 245 - 253
Date: 2014
Publisher: CLIN LAB PUBL
Place of Publication: HEIDELBERG
ISSN: 1433-6510
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
1ST LIVE BIRTH; FERTILITY PRESERVATION; CULTURE; CANCER; AUTOTRANSPLANTATION; STATISTICS; PREGNANCY; SURVIVAL; FAILURE; PATIENTMultiple languages
Medical Laboratory TechnologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/44951

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