Universität zu Köln

Pfeifer, Yvonne, Wilharm, Gottfried ORCID: 0000-0002-1771-6799, Zander, Esther, Wichelhaus, Thomas A., Goettig, Stefan, Hunfeld, Klaus-Peter, Seifert, Harald, Witte, Wolfgang and Higgins, Paul G. ORCID: 0000-0001-8677-9454 (2011). Molecular characterization of bla(NDM-1) in an Acinetobacter baumannii strain isolated in Germany in 2007. J. Antimicrob. Chemother., 66 (9). S. 1998 - 2002. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2091

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Abstract

Objectives: To investigate the genetic environment of the metallo-beta-lactamase gene bla(NDM-1) in an Acinetobacter baumannii isolated in 2007 in a German hospital. Methods: Antimicrobial susceptibility testing was performed and resistance genes were characterized by PCR amplification and sequencing. Transferability of beta-lactam resistance was tested by broth mating assays and transformation of plasmids. The genetic background of bla(NDM-1) was analysed by primer walking. Typing of the A. baumannii strain was performed by repetitive extragenic palindromic sequence-based PCR (rep-PCR) using the DiversiLab system. Results: The multidrug-resistant A. baumannii isolate harboured beta-lactamase genes bla(NDM-1) and intrinsic bla(OXA-64), but without the insertion sequence ISAba1 often located upstream. Transfer of carbapenem resistance by conjugation and transformation failed. Hybridization of isolated plasmid DNA with bla(NDM) probes was not successful. Shotgun cloning of whole genomic DNA and sequence analyses revealed that bla(NDM-1) was located between two insertion elements of ISAba125. Furthermore, this bla(NDM-1)-containing transposon structure was integrated into a chromosomal gene encoding a putative A. baumannii major facilitator superfamily (MFS) metabolite/H+ symporter. Conclusions: The metallo-beta-lactamase gene bla(NDM-1) in this A. baumannii strain was integrated in the chromosome on a new transposon structure composed of two copies of insertion sequence ISAba125. The variability of the genetic environment of bla(NDM-1) likely facilitates the rapid dissemination of this gene within many Gram-negative bacterial species.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pfeifer, Yvonne UNSPECIFIED UNSPECIFIED UNSPECIFIED Wilharm, Gottfried UNSPECIFIED orcid.org/0000-0002-1771-6799 UNSPECIFIED Zander, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED Wichelhaus, Thomas A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Goettig, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hunfeld, Klaus-Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Witte, Wolfgang UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-490339
DOI:	10.1093/jac/dkr256
Journal or Publication Title:	J. Antimicrob. Chemother.
Volume:	66
Number:	9
Page Range:	S. 1998 - 2002
Date:	2011
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2091
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language METALLO-BETA-LACTAMASE; MULTIPLEX PCR; GLOBAL SPREAD; RESISTANCE; EMERGENCE; GENES; CARBAPENEMASE Multiple languages Infectious Diseases; Microbiology; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49033