Universität zu Köln

Neumaier, Felix ORCID: 0000-0002-6376-6391, Kotliar, Konstantin, Haeren, Roel Hubert Louis, Temel, Yasin, Lueke, Jan Niklas, Seyam, Osama, Lindauer, Ute, Clusmann, Hans, Hescheler, Juergen, Schubert, Gerrit Alexander, Schneider, Toni and Albanna, Walid (2021). Retinal Vessel Responses to Flicker Stimulation Are Impaired in Ca(v)2.3-Deficient Mice-An in-vivo Evaluation Using Retinal Vessel Analysis (RVA). Front. Neurol., 12. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-2295

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Abstract

Objective: Metabolic demand increases with neuronal activity and adequate energy supply is ensured by neurovascular coupling (NVC). Impairments of NVC have been reported in the context of several diseases and may correlate with disease severity and outcome. Voltage-gated Ca2+-channels (VGCCs) are involved in the regulation of vasomotor tone. In the present study, we compared arterial and venous responses to flicker stimulation in Ca(v)2.3-competent (Ca(v)2.3([+/+])) and -deficient (Ca(v)2.3([-/-])) mice using retinal vessel analysis. Methods: The mice were anesthetized and the pupil of one eye was dilated by application of a mydriaticum. An adapted prototype of retinal vessel analyzer was used to perform dynamic retinal vessel analysis. Arterial and venous responses were quantified in terms of the area under the curve (AUC(art)/AUC(ven)) during flicker application, mean maximum dilation (mMD(art)/mMD(ven)) and time to maximum dilation (tMD(art)/tMD(ven)) during the flicker, dilation at flicker cessation (DFCart/DFCven), mean maximum constriction (mMC(art)/mMC(ven)), time to maximum constriction (tMC(art)/tMC(ven)) after the flicker and reactive magnitude (RMart/RMven). Results: A total of 33 retinal scans were conducted in 22 Ca(v)2.3([+/+]) and 11 Ca(v)2.3([-/-]) mice. Ca(v)2.3([-/-]) mice were characterized by attenuated and partially reversed arterial and venous responses, as reflected in significantly lower AUC(art) (p = 0.031) and AUC(ven) (p = 0.047), a trend toward reduced DFCart (p = 0.100), DFCven (p = 0.100), mMD(ven) (p = 0.075), and RMart (p = 0.090) and a trend toward increased tMD(art) (p = 0.096). Conclusion: To our knowledge, this is the first study using a novel, non-invasive analysis technique to document impairment of retinal vessel responses in VGCC-deficient mice. We propose that Ca(v)2.3 channels could be involved in NVC and may contribute to the impairment of vasomotor responses under pathophysiological conditions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Neumaier, Felix UNSPECIFIED orcid.org/0000-0002-6376-6391 UNSPECIFIED Kotliar, Konstantin UNSPECIFIED UNSPECIFIED UNSPECIFIED Haeren, Roel Hubert Louis UNSPECIFIED UNSPECIFIED UNSPECIFIED Temel, Yasin UNSPECIFIED UNSPECIFIED UNSPECIFIED Lueke, Jan Niklas UNSPECIFIED UNSPECIFIED UNSPECIFIED Seyam, Osama UNSPECIFIED UNSPECIFIED UNSPECIFIED Lindauer, Ute UNSPECIFIED UNSPECIFIED UNSPECIFIED Clusmann, Hans UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Schubert, Gerrit Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Toni UNSPECIFIED UNSPECIFIED UNSPECIFIED Albanna, Walid UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-590581
DOI:	10.3389/fneur.2021.659890
Journal or Publication Title:	Front. Neurol.
Volume:	12
Date:	2021
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-2295
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language B-WAVE AMPLITUDE; SUBARACHNOID HEMORRHAGE; NEUROVASCULAR UNIT; CALCIUM-CHANNELS; BLOOD-FLOW; AGE; CA(V)2.3; DIAMETER; LIGHT; RAT Multiple languages Clinical Neurology; Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59058