Ahmed, Shahira A., El-Mahallawy, Heba S., Mohamed, Samar Farag, Angelici, Maria Cristina, Hasapis, Kyriacos, Saber, Taisir and Karanis, Panagiotis (2022). Subtypes and phylogenetic analysis of Blastocystis sp. isolates from West Ismailia, Egypt. Sci Rep, 12 (1). BERLIN: NATURE PORTFOLIO. ISSN 2045-2322
Full text not available from this repository.Abstract
In Egypt, Blastocystis sp. is not yet on the diagnostic list of parasitology reports, and information about its subtypes (STs) is scarce. This study investigated its prevalence and its STs/alleles, performed phylogenetic analysis, and considered the distribution of risk factors associated with Blastocystis sp. infections in West Ismailia, Ismailia governorate. Sociodemographic data, exposure factors, and previous parasitic infection status were recorded for symptomatic and asymptomatic individuals. Microscopy, polymerase chain reaction, sequencing, and phylogenetic analysis for Blastocystis sp. isolated from fecal samples were performed. Eighty Blastocystis sp.-infected individuals (15.3%) were examined. The age of the individuals ranged between 0.60 and 85.0 (mean 17.10 +/- 15.70), the male/female ratio was 33/47, and the asymptomatic/symptomatic ratio was 55/25. The findings demonstrate clear evidence of direct contact with animals, poor water quality, and previous parasitic infections. Eleven samples yielded three Blastocystis STs (ST1: allele 4, ST2: alleles 9 and 12, and ST3: allele 34), with ST3 (45.5%) representing the most common subtype. Phylogenetic analysis with a robust bootstrap revealed three distinct clades for isolates of each subtype. This study updates the epidemiological knowledge of the distribution of Blastocystis sp. STs in Egypt and expands the current understanding of the prevalence, risk factor frequencies, and genetic diversity of this protist in the studied area.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-683722 | ||||||||||||||||||||||||||||||||
DOI: | 10.1038/s41598-022-23360-0 | ||||||||||||||||||||||||||||||||
Journal or Publication Title: | Sci Rep | ||||||||||||||||||||||||||||||||
Volume: | 12 | ||||||||||||||||||||||||||||||||
Number: | 1 | ||||||||||||||||||||||||||||||||
Date: | 2022 | ||||||||||||||||||||||||||||||||
Publisher: | NATURE PORTFOLIO | ||||||||||||||||||||||||||||||||
Place of Publication: | BERLIN | ||||||||||||||||||||||||||||||||
ISSN: | 2045-2322 | ||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/68372 |
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