Golumba-Nagy, Viktoria, Kuehle, Johannes and Abken, Hinrich (2017). Genetic Modification of T Cells with Chimeric Antigen Receptors: A Laboratory Manual. Hum. Gene Ther. Methods, 28 (6). S. 302 - 310. NEW ROCHELLE: MARY ANN LIEBERT, INC. ISSN 1946-6544
Full text not available from this repository.Abstract
Redirected T cells genetically modified with a chimeric antigen receptor (CAR) have induced spectacular remissions of refractory leukemia/lymphoma in early phase trials, attracting interest to use CAR T cells in a variety of other applications including solid cancer and nonmalignant diseases. However, extensive preclinical explorations demand highly effective and robust procedures for the genetic modification of blood T cells; the same applies for engineering with a recombinant T cell receptor. We present laboratory procedures in a step-by-step protocol to engineer human and mouse T cells with a CAR by -retro- or lentiviral transduction for further preclinical testing.
Item Type: | Journal Article | ||||||||||||||||
Creators: |
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URN: | urn:nbn:de:hbz:38-209045 | ||||||||||||||||
DOI: | 10.1089/hgtb.2017.083 | ||||||||||||||||
Journal or Publication Title: | Hum. Gene Ther. Methods | ||||||||||||||||
Volume: | 28 | ||||||||||||||||
Number: | 6 | ||||||||||||||||
Page Range: | S. 302 - 310 | ||||||||||||||||
Date: | 2017 | ||||||||||||||||
Publisher: | MARY ANN LIEBERT, INC | ||||||||||||||||
Place of Publication: | NEW ROCHELLE | ||||||||||||||||
ISSN: | 1946-6544 | ||||||||||||||||
Language: | English | ||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||
Subjects: | no entry | ||||||||||||||||
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Refereed: | Yes | ||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/20904 |
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