Universität zu Köln

Iebba, Valerio ORCID: 0000-0002-0716-306X, Santangelo, Floriana, Totino, Valentina, Pantanella, Fabrizio ORCID: 0000-0002-9612-968X, Monsia, Anatole, Di Cristanziano, Veronica, Di Cave, David, Schippa, Serena, Berrilli, Federica and D'Alfonso, Rossella (2016). Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Cote d'Ivoire. J. Infect. Dev. Ctries., 10 (9). S. 1035 - 1042. TRAMANIGLIO: J INFECTION DEVELOPING COUNTRIES. ISSN 1972-2680

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Abstract

Introduction: Literature data provide little information about protozoa infections and gut microbiota compositional shifts in humans. This preliminary study aimed to describe the fecal bacterial community composition of people from Cote d'Ivoire harboring Giardia duodenalis, Entamoeba spp., and Blastocystis hominis, in trying to discover possible alterations in their fecal microbiota structure related to the presence of such parasites. Methodology: Twenty fecal samples were collected from people inhabiting three different localities of Cote d'Ivoire for copromicroscopic analysis and molecular identification of G. duodenalis, Entamoeba spp., and B. hominis. Temporal temperature gradient gel electrophoresis (TTGE) was used to obtain a fingerprint of the overall bacterial community; quantitative polymerase chain reaction (qPCR) was used to define the relative abundances of selected bacterial species/group, and multivariate statistical analyses were employed to correlate all data. Results: Cluster analysis revealed a significant separation of TTGE profiles into four clusters (p < 0.0001), with a marked difference for G. duodenalis-positive samples in relation to the others (p = 5.4x10(-6)). Interestingly, qPCR data showed how G. duodenalis-positive samples were related to a dysbiotic condition that favors potentially harmful species (such as Escherichia coli), while Entamoeba spp./B. hominis-positive subjects were linked to a eubiotic condition, as shown by a significantly higher Faecalibacterium prausnitzii-Escherichia coli ratio. Conclusions: This preliminary investigation demonstrates a differential fecal microbiota structure in subjects infected with G. duodenalis or Entamoeba spp./B. hominis, paving the way for using further next-generation DNA technologies to better understand host-parasite-bacteria interactions, aimed at identifying potential indicators of microbiota changes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Iebba, Valerio UNSPECIFIED orcid.org/0000-0002-0716-306X UNSPECIFIED Santangelo, Floriana UNSPECIFIED UNSPECIFIED UNSPECIFIED Totino, Valentina UNSPECIFIED UNSPECIFIED UNSPECIFIED Pantanella, Fabrizio UNSPECIFIED orcid.org/0000-0002-9612-968X UNSPECIFIED Monsia, Anatole UNSPECIFIED UNSPECIFIED UNSPECIFIED Di Cristanziano, Veronica UNSPECIFIED UNSPECIFIED UNSPECIFIED Di Cave, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Schippa, Serena UNSPECIFIED UNSPECIFIED UNSPECIFIED Berrilli, Federica UNSPECIFIED UNSPECIFIED UNSPECIFIED D'Alfonso, Rossella UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-263114
DOI:	10.3855/jidc.8179
Journal or Publication Title:	J. Infect. Dev. Ctries.
Volume:	10
Number:	9
Page Range:	S. 1035 - 1042
Date:	2016
Publisher:	J INFECTION DEVELOPING COUNTRIES
Place of Publication:	TRAMANIGLIO
ISSN:	1972-2680
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REAL-TIME PCR; MINIMUM INFORMATION; PUBLICATION; COMMUNITIES; MICROFLORA; DIVERSITY; CHILDREN; IMPACT; DIET; MIQE Multiple languages Infectious Diseases Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26311