Al-Emran, Hassan M., Eibach, Daniel, Krumkamp, Ralf, Ali, Mohammad ORCID: 0000-0003-1410-388X, Baker, Stephen, Biggs, Holly M., Bjerregaard-Andersen, Morten, Breiman, Robert F., Clemens, John D., Crump, John A. ORCID: 0000-0002-4529-102X, Espinoza, Ligia Maria Cruz, Deerin, Jessica, Dekker, Denise Myriam, Gassama Sow, Amy, Hertz, Julian T., Im, Justin, Ibrango, Samuel, von Kalckreuth, Vera, Kabore, Leon Parfait, Konings, Frank, Lofberg, Sandra Valborg, Meyer, Christian G., Mintz, Eric D., Montgomery, Joel M., Olack, Beatrice, Pak, Gi Deok, Panzner, Ursula, Park, Se Eun, Razafindrabe, Jean Luco Tsiriniaina, Rabezanahary, Henintsoa, Rakotondrainiarivelo, Jean Philibert, Rakotozandrindrainy, Raphael, Raminosoa, Tiana Mirana, Schuett-Gerowitt, Heidi, Sampo, Emmanuel, Soura, Abdramane Bassiahi, Tall, Adama, Warren, Michelle, Wierzba, Thomas F., May, Juergen and Marks, Florian ORCID: 0000-0002-6043-7170 (2016). A Multicountry Molecular Analysis of Salmonella enterica Serovar Typhi With Reduced Susceptibility to Ciprofloxacin in Sub-Saharan Africa. Clin. Infect. Dis., 62. S. S42 - 5. CARY: OXFORD UNIV PRESS INC. ISSN 1537-6591

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Abstract

Methods.aEuro integral Febrile patients from 9 sites within 6 countries in SSA with a body temperature of a parts per thousand yen38.0A degrees C were enrolled in this study. Blood samples were obtained for bacterial culture, and Salmonella isolates were identified biochemically and confirmed by multiplex polymerase chain reaction (PCR). Antimicrobial susceptibility of all Salmonella isolates was performed by disk diffusion test, and minimum inhibitory concentrations (MICs) against ciprofloxacin were measured by Etest. All Salmonella isolates with reduced susceptibility to ciprofloxacin (MIC > 0.06 A mu g/mL) were screened for mutations in quinolone resistance-determining regions in target genes, and the presence of plasmid-mediated quinolone resistance (PMQR) genes was assessed by PCR. Results.aEuro integral A total of 8161 blood cultures were performed, and 100 (1.2%) S. Typhi, 2 (< 0.1%) Salmonella enterica serovar Paratyphi A, and 27 (0.3%) nontyphoid Salmonella (NTS) were isolated. Multidrug-resistant S. Typhi were isolated in Kenya (79% [n = 38]) and Tanzania (89% [n = 8]) only. Reduced ciprofloxacin-susceptible (22% [n = 11]) S. Typhi were isolated only in Kenya. Among those 11 isolates, all had a Glu133Gly mutation in the gyrA gene combined with either a gyrA (Ser83Phe) or gyrB mutation (Ser464Phe). One Salmonella Paratyphi A isolate with reduced susceptibility to ciprofloxacin was found in Senegal, with 1 mutation in gyrA (Ser83Phe) and a second mutation in parC (Ser57Phe). Mutations in the parE gene and PMQR genes were not detected in any isolate. Conclusions.aEuro integral Salmonella Typhi with reduced susceptibility to ciprofloxacin was not distributed homogenously throughout SSA. Its prevalence was very high in Kenya, and was not observed in other study countries. Continuous monitoring of antimicrobial susceptibility is required to follow the potential spread of antimicrobial-resistant isolates throughout SSA.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Al-Emran, Hassan M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eibach, DanielUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krumkamp, RalfUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ali, MohammadUNSPECIFIEDorcid.org/0000-0003-1410-388XUNSPECIFIED
Baker, StephenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Biggs, Holly M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bjerregaard-Andersen, MortenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Breiman, Robert F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Clemens, John D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Crump, John A.UNSPECIFIEDorcid.org/0000-0002-4529-102XUNSPECIFIED
Espinoza, Ligia Maria CruzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Deerin, JessicaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dekker, Denise MyriamUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gassama Sow, AmyUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hertz, Julian T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Im, JustinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ibrango, SamuelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
von Kalckreuth, VeraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kabore, Leon ParfaitUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Konings, FrankUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lofberg, Sandra ValborgUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meyer, Christian G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mintz, Eric D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Montgomery, Joel M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Olack, BeatriceUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pak, Gi DeokUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Panzner, UrsulaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Park, Se EunUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Razafindrabe, Jean Luco TsiriniainaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rabezanahary, HenintsoaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rakotondrainiarivelo, Jean PhilibertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rakotozandrindrainy, RaphaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Raminosoa, Tiana MiranaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schuett-Gerowitt, HeidiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sampo, EmmanuelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Soura, Abdramane BassiahiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tall, AdamaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Warren, MichelleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wierzba, Thomas F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
May, JuergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Marks, FlorianUNSPECIFIEDorcid.org/0000-0002-6043-7170UNSPECIFIED
URN: urn:nbn:de:hbz:38-281335
DOI: 10.1093/cid/civ788
Journal or Publication Title: Clin. Infect. Dis.
Volume: 62
Page Range: S. S42 - 5
Date: 2016
Publisher: OXFORD UNIV PRESS INC
Place of Publication: CARY
ISSN: 1537-6591
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GLOBAL BURDEN; RESISTANCE; MUTATIONS; PREVALENCE; PARATYPHI; DAKAR; QEPA; QNRMultiple languages
Immunology; Infectious Diseases; MicrobiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/28133

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