Universität zu Köln

Ocker, Ronja, Prompunjai, Yongyut, Chutipongvivate, Salakchit and Karanis, Panagiotis (2016). MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILAND. Rev. Inst. Med. Trop. Sao Paulo, 58. SAO PAULO: INST MEDICINA TROPICAL SAO PAULO. ISSN 1678-9946

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Abstract

The loop-mediated isothermal amplification method (LAMP) is a recently developed molecular technique that amplifies nucleic acid under isothermal conditions. For malaria diagnosis, 150 blood samples from consecutive febrile malaria patients, and healthy subjects were screened in Thailand. Each sample was diagnosed by LAMP, microscopy and nested polymerase chain reaction (nPCR), using nPCR as the gold standard. Malaria LAMP was performed using Plasmodium genus and Plasmodium falciparum specific assays in parallel. For the genus Plasmodium, microscopy showed a sensitivity and specificity of 100%, while LAMP presented 99% of sensitivity and 93% of specificity. For P. falciparum, microscopy had a sensitivity of 95%, and LAMP of 90%, regarding the specificity; and microscopy presented 93% and LAMP 97% of specificity. The results of the genus-specific LAMP technique were highly consistent with those of nPCR and the sensitivity of P. falciparum detection was only marginally lower.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ocker, Ronja UNSPECIFIED UNSPECIFIED UNSPECIFIED Prompunjai, Yongyut UNSPECIFIED UNSPECIFIED UNSPECIFIED Chutipongvivate, Salakchit UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-289487
DOI:	10.1590/S1678-9946201658027
Journal or Publication Title:	Rev. Inst. Med. Trop. Sao Paulo
Volume:	58
Date:	2016
Publisher:	INST MEDICINA TROPICAL SAO PAULO
Place of Publication:	SAO PAULO
ISSN:	1678-9946
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POLYMERASE-CHAIN-REACTION; FALCIPARUM-MALARIA; PLASMODIUM-VIVAX; VISUAL DETECTION; BLOOD; PCR; INFECTIONS; PARASITES; SENSITIVITY; MICROSCOPY Multiple languages Infectious Diseases; Parasitology; Tropical Medicine Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28948