Bernardes, Joana P., Mishra, Neha, Tran, Florian, Bahmer, Thomas, Best, Lena ORCID: 0000-0003-2772-153X, Blase, Johanna, I, Bordoni, Dora, Franzenburg, Jeanette, Geisen, Ulf ORCID: 0000-0002-6583-460X, Josephs-Spaulding, Jonathan ORCID: 0000-0001-9828-0685, Koehler, Philipp ORCID: 0000-0002-7386-7495, Kuenstner, Axel, Rosati, Elisa, Aschenbrenner, Anna C., Bacher, Petra, Baran, Nathan, Boysen, Teide, Brandt, Burkhard, Bruse, Niklas, Doerr, Jonathan, Draeger, Andreas, Elke, Gunnar, Ellinghaus, David, Fischer, Julia, Forster, Michael ORCID: 0000-0001-9927-5124, Franke, Andre, Franzenburg, Soeren, Frey, Norbert ORCID: 0000-0001-7611-378X, Friedrichs, Anette, Fuss, Janina, Glueck, Andreas, Hamm, Jacob, Hinrichsen, Finn, Hoeppner, Marc P., Imm, Simon, Junker, Ralf, Kaiser, Sina, Kan, Ying H., Knoll, Rainer, Lange, Christoph, Laue, Georg, Lier, Clemens, Lindner, Matthias, Marinos, Georgios, Markewitz, Robert, Nattermann, Jacob, Noth, Rainer, Pickkers, Peter, Rabe, Klaus F., Renz, Alina, Roecken, Christoph, Rupp, Jan, Schaffarzyk, Annika, Scheffold, Alexander ORCID: 0000-0002-0626-343X, Schulte-Schrepping, Jonas, Schunk, Domagoj, Skowasch, Dirk, Ulas, Thomas, Wandinger, Klaus-Peter, Wittig, Michael, Zimmermann, Johannes, Busch, Hauke, Hoyer, Bimba F., Kaleta, Christoph, Heyckendorf, Jan, Kox, Matthijs, Rybniker, Jan, Schreiber, Stefan, Schultze, Joachim L. and Rosenstiel, Philip
(2020).
Longitudinal Multi-omics Analyses Identify Responses of Megakaryocytes, Erythroid Cells, and Plasmablasts as Hallmarks of Severe COVID-19.
Immunity, 53 (6).
S. 1296 - 1324.
CAMBRIDGE:
CELL PRESS.
ISSN 1097-4180
Full text not available from this repository.
Abstract
Temporal resolution of cellular features associated with a severe COVID-19 disease trajectory is needed for understanding skewed immune responses and defining predictors of outcome. Here, we performed a longitudinal multi-omics study using a two-center cohort of 14 patients. We analyzed the bulk transcriptome, bulk DNA methylome, and single-cell transcriptome (>358,000 cells, including BCR profiles) of peripheral blood samples harvested from up to 5 time points. Validation was performed in two independent cohorts of COVID-19 patients. Severe COVID-19 was characterized by an increase of proliferating, metabolically hyperactive plasmablasts. Coinciding with critical illness, we also identified an expansion of interferon-activated circulating megakaryocytes and increased erythropoiesis with features of hypoxic signaling. Megakaryocyte- and erythroid-cell-derived co-expression modules were predictive of fatal disease outcome. The study demonstrates broad cellular effects of SARS-CoV-2 infection beyond adaptive immune cells and provides an entry point toward developing biomarkers and targeted treatments of patients with COVID-19.
Item Type: |
Journal Article
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Creators: |
Creators | Email | ORCID | ORCID Put Code |
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Bernardes, Joana P. | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Mishra, Neha | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Tran, Florian | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Bahmer, Thomas | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Best, Lena | UNSPECIFIED | orcid.org/0000-0003-2772-153X | UNSPECIFIED | Blase, Johanna, I | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Bordoni, Dora | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Franzenburg, Jeanette | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Geisen, Ulf | UNSPECIFIED | orcid.org/0000-0002-6583-460X | UNSPECIFIED | Josephs-Spaulding, Jonathan | UNSPECIFIED | orcid.org/0000-0001-9828-0685 | UNSPECIFIED | Koehler, Philipp | UNSPECIFIED | orcid.org/0000-0002-7386-7495 | UNSPECIFIED | Kuenstner, Axel | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Rosati, Elisa | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Aschenbrenner, Anna C. | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Bacher, Petra | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Baran, Nathan | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Boysen, Teide | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Brandt, Burkhard | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Bruse, Niklas | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Doerr, Jonathan | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Draeger, Andreas | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Elke, Gunnar | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Ellinghaus, David | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Fischer, Julia | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Forster, Michael | UNSPECIFIED | orcid.org/0000-0001-9927-5124 | UNSPECIFIED | Franke, Andre | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Franzenburg, Soeren | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Frey, Norbert | UNSPECIFIED | orcid.org/0000-0001-7611-378X | UNSPECIFIED | Friedrichs, Anette | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Fuss, Janina | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Glueck, Andreas | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Hamm, Jacob | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Hinrichsen, Finn | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Hoeppner, Marc P. | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Imm, Simon | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Junker, Ralf | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Kaiser, Sina | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Kan, Ying H. | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Knoll, Rainer | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Lange, Christoph | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Laue, Georg | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Lier, Clemens | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Lindner, Matthias | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Marinos, Georgios | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Markewitz, Robert | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Nattermann, Jacob | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Noth, Rainer | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Pickkers, Peter | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Rabe, Klaus F. | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Renz, Alina | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Roecken, Christoph | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Rupp, Jan | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Schaffarzyk, Annika | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Scheffold, Alexander | UNSPECIFIED | orcid.org/0000-0002-0626-343X | UNSPECIFIED | Schulte-Schrepping, Jonas | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Schunk, Domagoj | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Skowasch, Dirk | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Ulas, Thomas | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Wandinger, Klaus-Peter | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Wittig, Michael | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Zimmermann, Johannes | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Busch, Hauke | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Hoyer, Bimba F. | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Kaleta, Christoph | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Heyckendorf, Jan | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Kox, Matthijs | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Rybniker, Jan | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Schreiber, Stefan | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Schultze, Joachim L. | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED | Rosenstiel, Philip | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED |
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URN: |
urn:nbn:de:hbz:38-307993 |
DOI: |
10.1016/j.immuni.2020.11.017 |
Journal or Publication Title: |
Immunity |
Volume: |
53 |
Number: |
6 |
Page Range: |
S. 1296 - 1324 |
Date: |
2020 |
Publisher: |
CELL PRESS |
Place of Publication: |
CAMBRIDGE |
ISSN: |
1097-4180 |
Language: |
English |
Faculty: |
Unspecified |
Divisions: |
Unspecified |
Subjects: |
no entry |
Uncontrolled Keywords: |
Keywords | Language |
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HEMATOPOIETIC STEM; INNATE IMMUNITY; HYPOXIA; DATABASE; DIFFERENTIATION; RECONSTRUCTION; INHIBITION; METABOLISM; EXPRESSION; PLATELETS | Multiple languages | Immunology | Multiple languages |
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URI: |
http://kups.ub.uni-koeln.de/id/eprint/30799 |
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