Universität zu Köln

Kusch, Stephanie ORCID: 0000-0002-2708-4975, Jaeschke, Andrea, Moerchen, Ramona and Rethemeyer, Janet (2020). Tracing life at the dry limit using phospholipid fatty acids - does sampling matter? Soil Biol. Biochem., 141. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 0038-0717

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Abstract

Phospholipid fatty acids (PLFAs) have been used to trace bacterial life in extremely carbon-poor soils of the hyperarid Atacama Desert. However, the low abundances of bacteria and, thus, PLFAs increases the risk of contamination by exogenous PLFAs. Here, we assess whether field sampling strategies (super-clean, clean, and regular sampling protocols) have an effect on PLFA diversity and abundance in hyperarid Atacama soils or whether laboratory processing or true environmental heterogeneity control PLFA inventories. Our results show no exogenous PLFA contribution during sample processing in the lab and statistical analyses (ANOVA, Kruskal-Wallis) reveal that PLFA abundances do not differ significantly between replicate samples (n = 3) taken with the three different sampling strategies. Rather than sampling strategy, our results show that PLFA abundances in the investigated soil replicates rather reflect true environmental heterogeneity of primarily bacterial biomass (in the absence of indigenous fungi), potentially related to small-scale physicochemical differences.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kusch, Stephanie UNSPECIFIED orcid.org/0000-0002-2708-4975 UNSPECIFIED Jaeschke, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Moerchen, Ramona UNSPECIFIED UNSPECIFIED UNSPECIFIED Rethemeyer, Janet UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-347149
DOI:	10.1016/j.soilbio.2019.107661
Journal or Publication Title:	Soil Biol. Biochem.
Volume:	141
Date:	2020
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	0038-0717
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARID ATACAMA DESERT; MICROBIAL COMMUNITIES; SOIL; DIVERSITY; RATIO Multiple languages Soil Science Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34714