Refaian, Nasrin, Schlereth, Simona L., Koch, Konrad R., Notara, Maria, Hos, Deniz, Mescher, Melina, Iden, Sandra ORCID: 0000-0003-2333-9827, Bosch, Jacobus J., Jager, Martine J., Cursiefen, Claus and Heindl, Ludwig M. (2015). Comparing the Hem- and Lymphangiogenic Profile of Conjunctival and Uveal Melanoma Cell Lines. Invest. Ophthalmol. Vis. Sci., 56 (9). S. 5691 - 5698. ROCKVILLE: ASSOC RESEARCH VISION OPHTHALMOLOGY INC. ISSN 1552-5783

Full text not available from this repository.

Abstract

PURPOSE. Malignant melanomas of the ocular surface (conjunctival melanoma [CM]) and within the eye (uveal melanoma [UM]) show different types of metastatic behavior. While CM has a propensity to spread first to regional lymph nodes, UM metastasizes almost exclusively via the hematogenic route to the liver. We investigated whether these different metastatic patterns might be attributable to differential hem-and lymphangiogenic characteristics of CM and UM cells. METHODS. Human CM (CM2005.1, CRMM1, CRMM2) and UM (Mel270, Mel290, OM431) cell lines were analyzed for VEGF-A, -C, and -D expression by RT-PCR and ELISA. The influence of CM-or UM-conditioned medium on blood (BEC) and lymphatic (LEC) endothelial cell proliferation and migration was measured using 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide (MTT) and scratch assays, respectively. RESULTS. Vascular endothelial growth factor-A, -C and -D mRNA, and VEGF-A and -D protein were expressed by all CM and UM cell lines, while VEGF-C protein was only expressed by UM cell lines. The CM-and UM-conditioned medium did neither differentially affect BEC (P = 0.86) and LEC (P = 0.90) proliferation, nor BEC (P = 0.56) and LEC (P = 0.90) migration. CONCLUSIONS. Conjunctival melanoma cell lines did not show a higher prolymphangiogenic potential, and UM cell lines did not show a higher prohemangiogenic potential. Accordingly, other mechanisms within the tumor microenvironment might account for the diverging metastatic patterns of conjunctival versus uveal melanomas.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Refaian, NasrinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schlereth, Simona L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koch, Konrad R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Notara, MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hos, DenizUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mescher, MelinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Iden, SandraUNSPECIFIEDorcid.org/0000-0003-2333-9827UNSPECIFIED
Bosch, Jacobus J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jager, Martine J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cursiefen, ClausUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heindl, Ludwig M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-396836
DOI: 10.1167/iovs.15-16829
Journal or Publication Title: Invest. Ophthalmol. Vis. Sci.
Volume: 56
Number: 9
Page Range: S. 5691 - 5698
Date: 2015
Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Place of Publication: ROCKVILLE
ISSN: 1552-5783
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ENDOTHELIAL-GROWTH-FACTOR; TUMOR-ASSOCIATED LYMPHANGIOGENESIS; AMERICAN JOINT COMMITTEE; CILIARY-BODY-MELANOMAS; MALIGNANT MELANOMAS; FACTOR-A; VEGF-A; CANCER; ANGIOGENESIS; EXPRESSIONMultiple languages
OphthalmologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/39683

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item