Universität zu Köln

Warren, Ben ORCID: 0000-0002-7871-0091 and Kloppenburg, Peter (2014). Rapid and Slow Chemical Synaptic Interactions of Cholinergic Projection Neurons and GABAergic Local Interneurons in the Insect Antennal Lobe. J. Neurosci., 34 (39). S. 13039 - 13047. WASHINGTON: SOC NEUROSCIENCE. ISSN 0270-6474

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Abstract

The antennal lobe (AL) of insects constitutes the first synaptic relay and processing center of olfactory information, received from olfactory sensory neurons located on the antennae. Complex synaptic connectivity between olfactory neurons of the AL ultimately determines the spatial and temporal tuning profile of (output) projection neurons to odors. Here we used paired whole-cell patch-clamp recordings in the cockroach Periplaneta americana to characterize synaptic interactions between cholinergic uniglomerular projection neurons (uPNs) and GABAergic local interneurons (LNs), both of which are key components of the insect olfactory system. We found rapid, strong excitatory synaptic connections between uPNs and LNs. This rapid excitatory transmission was blocked by the nicotinic acetylcholine receptor blocker mecamylamine. IPSPs, elicited by synaptic input from a presynaptic LN, were recorded in both uPNs and LNs. IPSPs were composed of both slow, sustained components and fast, transient components which were coincident with presynaptic action potentials. The fast IPSPs were blocked by the GABA(A) receptor chloride channel blocker picrotoxin, whereas the slow sustained IPSPs were blocked by the GABA(B) receptor blocker CGP-54626. This is the first study to directly show the predicted dual fast-and slow-inhibitory action of LNs, which was predicted to be key in shaping complex odor responses in the AL of insects. We also provide the first direct characterization of rapid postsynaptic potentials coincident with presynaptic spikes between olfactory processing neurons in the AL.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Warren, Ben UNSPECIFIED orcid.org/0000-0002-7871-0091 UNSPECIFIED Kloppenburg, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-428486
DOI:	10.1523/JNEUROSCI.0765-14.2014
Journal or Publication Title:	J. Neurosci.
Volume:	34
Number:	39
Page Range:	S. 13039 - 13047
Date:	2014
Publisher:	SOC NEUROSCIENCE
Place of Publication:	WASHINGTON
ISSN:	0270-6474
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MOTH MANDUCA-SEXTA; GABA-IMMUNOREACTIVE NEURONS; PERIPLANETA-AMERICANA; MORPHOLOGICAL CHARACTERIZATION; OLFACTORY GLOMERULI; NETWORK MECHANISMS; MUSHROOM BODIES; COCKROACH; HONEYBEE; ORGANIZATION Multiple languages Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42848