Universität zu Köln

Wang, Mengying, Todorov, Plamen, Isachenko, Evgenia, Rahimi, Gohar, Wang, Wanxue ORCID: 0000-0003-0606-6628, von Brandenstein, Melanie, Kumar, Pradeep, Mallmann, Peter and Isachenko, Vladimir (2021). Aseptic capillary vitrification of human spermatozoa: Cryoprotectant-free vs. cryoprotectant-included technologies. Cryobiology, 99. S. 95 - 103. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1090-2392

Full text not available from this repository.

Abstract

The protocol of aseptic cryoprotectant-free vitrification on human spermatozoa is well documented. However, data about the effect of permeable cryoprotectants at this procedure is limited. Presented study aimed to test the aseptic capillary vitrification technologies using permeable cryoprotectant-included or cryopmtectant-free media. Thirty-two normal samples were included and analyzed after vitrification in three different media and thawing. Three treatment groups were formed: Group 1, basic medium; Group 2, basic medium with 0.25 M sucrose; Group 3, basic medium with glycerol. Before plunging into liquid nitrogen, capillaries were filled by 10 til of spermatozoa suspension and isolated from liquid nitrogen by location in hermetically closed 0.25 ml straws. Progressive motility, plasma membrane integrity, total motility/viability after 24, 48 and 72 h in vitro culture, apoptosis and mitochondrial membrane potential (Delta Psi m) were determined after thawing at 42 degrees C. Progressive motility of spermatozoa in groups 1, 2, 3 was 24.9 +/- 1.7%, 34.5 +/- 2.8% and 34.0 +/- 1.4%, respectively (P-1-2,P-3<0.05). The plasma membrane integrity of spermatozoa in groups 2 and 3 (48.4 +/- 2.9% and 45.5 +/- 3.9%, respectively) was higher than in Group 1 (33.3 +/- 2.1%, P < 0.05). After 24 h, 48 h and 72 h in vitro culture, the total motility and viability of spermatozoa in Group 1 was significantly lower than Group 2 and Group 3. The apoptosis rate in Group 3 (44.5 +/- 3.0%) and Group 2 (47.7 +/- 4.1%) were lower than in Group 1 (52.5 +/- 4.4%; P < 0.05). Delta Psi m rates in Group 3 and Group 2 were higher than in Group 1 (P < 0.05) with no statistical differences between this parameter in Group 2 and Group 3 (P > 0.1). In conclusion, supplementation of medium for aseptic capillary technology for cryoprotectant-free vitrification of human spermatozoa by permeable cryoprotectant does not improve the quality of spermatozoa after warming.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wang, Mengying UNSPECIFIED UNSPECIFIED UNSPECIFIED Todorov, Plamen UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahimi, Gohar UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Wanxue UNSPECIFIED orcid.org/0000-0003-0606-6628 UNSPECIFIED von Brandenstein, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Kumar, Pradeep UNSPECIFIED UNSPECIFIED UNSPECIFIED Mallmann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Vladimir UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-573817
DOI:	10.1016/j.cryobiol.2021.01.006
Journal or Publication Title:	Cryobiology
Volume:	99
Page Range:	S. 95 - 103
Date:	2021
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1090-2392
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CRYOPRESERVED HUMAN SPERM; FERTILIZING-CAPACITY; WATER PERMEABILITY; MOTILITY; MORPHOLOGY; SURVIVAL; FROZEN; QUALITY; PARAMETERS; INTEGRITY Multiple languages Biology; Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57381