Schlachtenberger, Georg ORCID: 0000-0001-7118-8432, Doerr, Fabian, Brezina, Annamaria, Menghesha, Hruy, Heldwein, Matthias B., Bennink, Gerardus, Menger, Michael D., Moussavian, Mohammed, Hekmat, Khosro and Wahlers, Thorsten (2021). Perigraft reaction and incorporation of porcine and bovine pericardial patches. Interact Cardiovasc. Thorac. Surg., 32 (4). S. 638 - 648. OXFORD: OXFORD UNIV PRESS. ISSN 1569-9285

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Abstract

OBJECTIVES: Bovine and porcine pericardial patches are frequently used in cardiothoracic and vascular surgery. There are no guidelines recommending the usage of these patches for particular surgical approaches. However, these 2 materials supposedly possess different properties. The clinical advantage of porcine compared with bovine patches remains controversial. In this experimental study, we analysed the incorporation and vascularization of bovine and porcine pericardial patches during the initial phase after implantation. METHODS: Bovine and porcine pericardial patches were implanted into the dorsal skinfold chamber of C57BL/6 mice (n = 8 per group) to study vascularization and inflammation at the implantation site using repetitive intravital fluorescence microscopy over a 14-day period. At the end of the in vivo experiments, CD-31-positive cells were determined to evaluate the vascularization by immunohistochemistry. Furthermore, cell proliferation and apoptosis were analysed immunohistochemically. RESULTS: Implanted bovine patches exhibited an enhanced vascularization, as indicated by a significantly higher number of CD-31-positive cells and micro-vessels (23.2 +/- 4.3 vs 16.5 +/- 5.8 mm(-2); P=0.001). Furthermore, bovine patches showed a slightly but not significantly higher functional capillary density. Both patches induced a moderate leukocytic inflammatory host tissue response, and neither bovine nor porcine patches significantly affected apoptosis and cell proliferation at the implantation site. CONCLUSIONS: Bovine and porcine pericardial patches are similarly suitable for surgery. Bovine patches exhibited an improved vascularization during the first 14 days after implantation. This may result in a quicker and improved incorporation into the surrounding tissue compared with porcine pericardial patches.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Schlachtenberger, GeorgUNSPECIFIEDorcid.org/0000-0001-7118-8432UNSPECIFIED
Doerr, FabianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brezina, AnnamariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Menghesha, HruyUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heldwein, Matthias B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bennink, GerardusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Menger, Michael D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Moussavian, MohammedUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hekmat, KhosroUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wahlers, ThorstenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-578385
DOI: 10.1093/icvts/ivaa308
Journal or Publication Title: Interact Cardiovasc. Thorac. Surg.
Volume: 32
Number: 4
Page Range: S. 638 - 648
Date: 2021
Publisher: OXFORD UNIV PRESS
Place of Publication: OXFORD
ISSN: 1569-9285
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PULMONARY-ARTERY; IMMUNE-RESPONSE; VASCULAR GRAFTS; AORTIC-VALVE; RECONSTRUCTION; METAANALYSIS; PROSTHESES; ACETATE; SILVERMultiple languages
Cardiac & Cardiovascular Systems; Respiratory System; SurgeryMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/57838

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