Universität zu Köln

Zuo, Weiliang ORCID: 0000-0001-5241-1440, Depotter, Jasper R. L., Gupta, Deepak K., Thines, Marco and Doehlemann, Gunther ORCID: 0000-0002-7353-8456 (2021). Cross-species analysis between the maize smut fungi Ustilago maydis and Sporisorium reilianum highlights the role of transcriptional change of effector orthologs for virulence and disease. New Phytol., 232 (2). S. 719 - 734. HOBOKEN: WILEY. ISSN 1469-8137

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Abstract

The constitution and regulation of effector repertoires shape host-microbe interactions. Ustilago maydis and Sporisorium reilianum are two closely related smut fungi, which both infect maize but cause distinct disease symptoms. Understanding how effector orthologs are regulated in these two pathogens can therefore provide insights into the evolution of different infection strategies. We tracked the infection progress of U. maydis and S. reilianum in maize leaves and used two distinct infection stages for cross-species RNA-sequencing analyses. We identified 207 of 335 one-to-one effector orthologs as differentially regulated during host colonization, which might reflect the distinct disease development strategies. Using CRISPR-Cas9-mediated gene conversion, we identified two differentially expressed effector orthologs with conserved function between two pathogens. Thus, differential expression of functionally conserved genes might contribute to species-specific adaptation and symptom development. Interestingly, another differentially expressed orthogroup (UMAG_05318/Sr10075) showed divergent protein function, providing a possible case for neofunctionalization. Collectively, we demonstrated that the diversification of effector genes in related pathogens can be caused both by alteration on the transcriptional level and through functional diversification of the encoded effector proteins.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zuo, Weiliang UNSPECIFIED orcid.org/0000-0001-5241-1440 UNSPECIFIED Depotter, Jasper R. L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gupta, Deepak K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Thines, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED Doehlemann, Gunther UNSPECIFIED orcid.org/0000-0002-7353-8456 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-586777
DOI:	10.1111/nph.17625
Journal or Publication Title:	New Phytol.
Volume:	232
Number:	2
Page Range:	S. 719 - 734
Date:	2021
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-8137
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GENOME EVOLUTION; PLANT-PATHOGENS; PROTEIN; GENES; HOST; COLONIZATION; ALIGNMENT; DEFENSES; CYCLE Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58677