Esmaillie, Reza, Ignarski, Michael ORCID: 0000-0001-6057-7694, Bohl, Katrin, Krueger, Tim, Ahmad, Daniyal, Seufert, Lisa, Schermer, Bernhard, Benzing, Thomas, Mueller, Roman-Ulrich and Fabretti, Francesca (2019). Activation of Hypoxia-Inducible Factor Signaling Modulates the RNA Protein Interactome in Caenorhabditis elegans. iScience, 22. S. 466 - 492. CAMBRIDGE: CELL PRESS. ISSN 2589-0042

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Abstract

The cellular response to hypoxia is crucial to organismal survival, and hypoxia-inducible factors (HIF) are the key mediators of this response. HIF-signaling is central to many human diseases and mediates longevity in the nematode. Despite the rapidly increasing knowledge on RNA-binding proteins (RBPs), little is known about their contribution to hypoxia-induced cellular adaptation. We used RNA interactome capture (RIC) in wild-type Caenorhabditis elegans and vhl-1 loss-of-function mutants to fill this gap. This approach identifies more than 1,300 nematode RBPs, 270 of which can be considered novel RBPs. Interestingly, loss of vhl-1 modulates the RBPome. This difference is not primarily explained by protein abundance suggesting differential RNA-binding. Taken together, our study provides a global view on the nematode RBPome and proteome as well as their modulation by HIF-signaling. The resulting RBP atlas is also provided as an interactive online data mining tool (http://shiny.cecad.uni-koeln.de:3838/celegans_rbpome).

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Esmaillie, RezaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ignarski, MichaelUNSPECIFIEDorcid.org/0000-0001-6057-7694UNSPECIFIED
Bohl, KatrinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krueger, TimUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ahmad, DaniyalUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Seufert, LisaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schermer, BernhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Benzing, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mueller, Roman-UlrichUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fabretti, FrancescaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-124153
DOI: 10.1016/j.isci.2019.11.039
Journal or Publication Title: iScience
Volume: 22
Page Range: S. 466 - 492
Date: 2019
Publisher: CELL PRESS
Place of Publication: CAMBRIDGE
ISSN: 2589-0042
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
TUMOR-SUPPRESSOR PROTEIN; BINDING PROTEINS; BOUND PROTEOME; GENOME-WIDE; IDENTIFICATION; RECOGNITION; TRANSLATION; CHAPERONIN; COMPLEX; TARGETSMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/12415

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