Universität zu Köln

Esmaillie, Reza, Ignarski, Michael ORCID: 0000-0001-6057-7694, Bohl, Katrin, Krueger, Tim, Ahmad, Daniyal, Seufert, Lisa, Schermer, Bernhard, Benzing, Thomas, Mueller, Roman-Ulrich and Fabretti, Francesca (2019). Activation of Hypoxia-Inducible Factor Signaling Modulates the RNA Protein Interactome in Caenorhabditis elegans. iScience, 22. S. 466 - 492. CAMBRIDGE: CELL PRESS. ISSN 2589-0042

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Abstract

The cellular response to hypoxia is crucial to organismal survival, and hypoxia-inducible factors (HIF) are the key mediators of this response. HIF-signaling is central to many human diseases and mediates longevity in the nematode. Despite the rapidly increasing knowledge on RNA-binding proteins (RBPs), little is known about their contribution to hypoxia-induced cellular adaptation. We used RNA interactome capture (RIC) in wild-type Caenorhabditis elegans and vhl-1 loss-of-function mutants to fill this gap. This approach identifies more than 1,300 nematode RBPs, 270 of which can be considered novel RBPs. Interestingly, loss of vhl-1 modulates the RBPome. This difference is not primarily explained by protein abundance suggesting differential RNA-binding. Taken together, our study provides a global view on the nematode RBPome and proteome as well as their modulation by HIF-signaling. The resulting RBP atlas is also provided as an interactive online data mining tool (http://shiny.cecad.uni-koeln.de:3838/celegans_rbpome).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Esmaillie, Reza UNSPECIFIED UNSPECIFIED UNSPECIFIED Ignarski, Michael UNSPECIFIED orcid.org/0000-0001-6057-7694 UNSPECIFIED Bohl, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Tim UNSPECIFIED UNSPECIFIED UNSPECIFIED Ahmad, Daniyal UNSPECIFIED UNSPECIFIED UNSPECIFIED Seufert, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Schermer, Bernhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Benzing, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Roman-Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Fabretti, Francesca UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-124153
DOI:	10.1016/j.isci.2019.11.039
Journal or Publication Title:	iScience
Volume:	22
Page Range:	S. 466 - 492
Date:	2019
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2589-0042
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TUMOR-SUPPRESSOR PROTEIN; BINDING PROTEINS; BOUND PROTEOME; GENOME-WIDE; IDENTIFICATION; RECOGNITION; TRANSLATION; CHAPERONIN; COMPLEX; TARGETS Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12415