Toy, Trevor, Pak, Gi Deok, Campbell, James I., El Tayeb, Muna Ahmed, Von Kalckreuth, Vera, Im, Justin, Panzner, Ursula, Espinoza, Ligia Maria Cruz, Eibach, Daniel, Dekker, Denise Myriam, Park, Se Eun, Jeon, Hyon Jin, Konings, Frank, Mogeni, Ondari D., Cosmas, Leonard, Bjerregaard-Andersen, Morten, Gasmelseed, Nagla, Hertz, Julian T., Jaeger, Anna, Krumkamp, Ralf, Ley, Benedikt, Thriemer, Kamala, Kabore, Leon Parfait, Niang, Aissatou, Raminosoa, Tiana Mirana, Sampo, Emmanuel, Sarpong, Nimako, Soura, Abdramane, Owusu-Dabo, Ellis, Teferi, Mekonnen, Yeshitela, Biruk, Poppert, Sven, May, Juergen, Kim, Jerome H., Chon, Yun, Park, Jin Kyung, Aseffa, Abroaham, Breiman, Robert F., Schuett-Gerowitt, Heidi, Aaby, Peter, Adu-Sarkodie, Yaw, Crump, John A., Rakotozandrindrainy, Raphael, Meyer, Christian G., Sow, Amy Gassama, Clemens, John D., Wierzba, Thomas F., Baker, Stephen and Marks, Florian (2019). Multicountry Distribution and Characterization of Extended-spectrum beta-Lactamase-associated Gram-negative Bacteria From Bloodstream Infections in Sub-Saharan Africa. Clin. Infect. Dis., 69. S. S449 - 10. CARY: OXFORD UNIV PRESS INC. ISSN 1537-6591

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Abstract

Background. Antimicrobial resistance (AMR) is a major global health concern, yet, there are noticeable gaps in AMR surveillance data in regions such as sub-Saharan Africa. We aimed to measure the prevalence of extended-spectrum beta-lactamase (ESBL) producing Gram-negative bacteria in bloodstream infections from 12 sentinel sites in sub-Saharan Africa. Methods. Data were generated during the Typhoid Fever Surveillance in Africa Program (TSAP), in which standardized blood cultures were performed on febrile patients attending 12 health facilities in 9 sub-Saharan African countries between 2010 and 2014. Pathogenic bloodstream isolates were identified at the sites and then subsequently confirmed at a central reference laboratory. Antimicrobial susceptibility testing, detection of ESBL production, and conventional multiplex polymerase chain reaction (PCR) testing for genes encoding for beta-lactamase were performed on all pathogens. Results. Five hundred and five pathogenic Gram-negative bloodstream isolates were isolated during the study period and available for further characterization. This included 423 Enterobacteriaceae. Phenotypically, 61 (12.1%) isolates exhibited ESBL activity, and genotypically, 47 (9.3%) yielded a PCR amplicon for at least one of the screened ESBL genes. Among specific Gram-negative isolates, 40 (45.5%) of 88 Klebsiella spp., 7 (5.7%) of 122 Escherichia coli, 6 (16.2%) of 37 Acinetobacter spp., and 2 (1.3%) of 159 of nontyphoidal Salmonella (NTS) showed phenotypic ESBL activity. Conclusions. Our findings confirm the presence of ESBL production among pathogens causing bloodstream infections in sub-Saharan Africa. With few alternatives for managing ESBL-producing pathogens in the African setting, measures to control the development and proliferation of AMR organisms are urgently needed.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Toy, TrevorUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pak, Gi DeokUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Campbell, James I.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
El Tayeb, Muna AhmedUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Von Kalckreuth, VeraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Im, JustinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Panzner, UrsulaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Espinoza, Ligia Maria CruzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eibach, DanielUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dekker, Denise MyriamUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Park, Se EunUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jeon, Hyon JinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Konings, FrankUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mogeni, Ondari D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cosmas, LeonardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bjerregaard-Andersen, MortenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gasmelseed, NaglaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hertz, Julian T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jaeger, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krumkamp, RalfUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ley, BenediktUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thriemer, KamalaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kabore, Leon ParfaitUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Niang, AissatouUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Raminosoa, Tiana MiranaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sampo, EmmanuelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sarpong, NimakoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Soura, AbdramaneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Owusu-Dabo, EllisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Teferi, MekonnenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yeshitela, BirukUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Poppert, SvenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
May, JuergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kim, Jerome H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chon, YunUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Park, Jin KyungUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Aseffa, AbroahamUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Breiman, Robert F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schuett-Gerowitt, HeidiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Aaby, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Adu-Sarkodie, YawUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Crump, John A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rakotozandrindrainy, RaphaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meyer, Christian G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sow, Amy GassamaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Clemens, John D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wierzba, Thomas F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Baker, StephenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Marks, FlorianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-127563
DOI: 10.1093/cid/ciz450
Journal or Publication Title: Clin. Infect. Dis.
Volume: 69
Page Range: S. S449 - 10
Date: 2019
Publisher: OXFORD UNIV PRESS INC
Place of Publication: CARY
ISSN: 1537-6591
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
MULTIPLEX PCR; GENESMultiple languages
Immunology; Infectious Diseases; MicrobiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/12756

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