Universität zu Köln

Scherf-Clavel, Oliver ORCID: 0000-0002-1967-9231, Kinzig, Martina, Stoffel, Marc S., Fuhr, Uwe and Soergel, Fritz (2019). A HILIC-MS/MS assay for the quantification of metformin and sitagliptin in human plasma and urine: A tool for studying drug transporter perturbation. J. Pharm. Biomed. Anal., 175. AMSTERDAM: ELSEVIER. ISSN 1873-264X

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Abstract

This article describes the development and validation of a HILIC-MS/MS method for the simultaneous quantification of metformin and sitagliptin from human plasma and urine. The presented method uses quick sample preparation and fast chromatography allowing for high sample throughput. The quantification is performed using multi-reaction monitoring and ESI positive mode with stable isotope labelled internal standards for both metformin and sitagliptin. Excellent linearity in the selected calibrations ranges, low inter-day variability (CV% <6.7%), and high accuracy (95.5-104.1%) were obtained. Adequate retention was attained for both analytes by hydrophilic interaction liquid chromatography using a plain silica column in combination with a mobile phase composed of ammonium formate, acetonitrile, formic acid and water in gradient separation mode. (C) 2019 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Scherf-Clavel, Oliver UNSPECIFIED orcid.org/0000-0002-1967-9231 UNSPECIFIED Kinzig, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Stoffel, Marc S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fuhr, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Soergel, Fritz UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-130414
DOI:	10.1016/j.jpba.2019.07.002
Journal or Publication Title:	J. Pharm. Biomed. Anal.
Volume:	175
Date:	2019
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1873-264X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TANDEM MASS-SPECTROMETRY; ONLINE EXTRACTION; HUMAN SERUM; LC-MS/MS; GLIMEPIRIDE; INHIBITOR; VALIDATION; MULTIDRUG; MS Multiple languages Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13041