Universität zu Köln

Spis, Emilia, Bushkovskaia, Anna, Isachenko, Evgenia, Todorov, Plamen ORCID: 0000-0003-3731-6116, Sanchez, Raul, Skopets, Viola and Isachenko, Vladimir (2019). Conventional freezing vs. cryoprotectant-free vitrification of epididymal (MESA) and testicular (TESE) spermatozoa: Three live births. Cryobiology, 90. S. 100 - 103. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1090-2392

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Abstract

Data of cryoprotectant-free vitrification of human testicular and epididymal spermatozoa are limited. The aim of this investigation was to compare two aseptic technologies of TESE (testicular) and MESA (epididymal) spermatozoa cryopreservation: standard conventional freezing with the use of cryoprotectants and cryoprotectantfree vitrification. Sperm motility, capacitation-like changes, acrosome reaction and the mitochondria] membrane potential of frozen (5% glycerol, 10 C/min) and vitrified (Human Tubal Fluid + 1% Human Serum Albumin + 0.25 M sucrose, plunging into liquid nitrogen of capillaries with spermatozoa isolated from liquid nitrogen (aseptic method) were compared. The quality of the cryoprotectant-free vitrified MESA- and TESE-spermatozoa was higher than that of spermatozoa conventionally frozen with permeable cryoprotectants. Intracellular sperm injection (ICSI) was performed with vitrified spermatozoa. We report the birth of three healthy babies from two women following ICSI with motile MESA- and TESE-spermatozoa vitrified without cryoprotectants. This is the first report of full -term pregnancies and babies born after ICSI with epididymal and testicular spermatozoa vitrified without cryoprotectants. In conclusion, cryoprotectant-free vitrification can be successfully applied for the cryopreservation of motile TESE- and MESA-spermatozoa.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Spis, Emilia UNSPECIFIED UNSPECIFIED UNSPECIFIED Bushkovskaia, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Todorov, Plamen UNSPECIFIED orcid.org/0000-0003-3731-6116 UNSPECIFIED Sanchez, Raul UNSPECIFIED UNSPECIFIED UNSPECIFIED Skopets, Viola UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Vladimir UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-132321
DOI:	10.1016/j.cryobiol.2019.08.003
Journal or Publication Title:	Cryobiology
Volume:	90
Page Range:	S. 100 - 103
Date:	2019
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1090-2392
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VITRO FERTILIZATION; INJECTION Multiple languages Biology; Physiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13232