Universität zu Köln

Geisler, Stefanie, Stegmayr, Carina ORCID: 0000-0003-4191-8734, Niemitz, Nicole, Lohmann, Philipp ORCID: 0000-0002-5360-046X, Rapp, Marion, Stoffels, Gabriele, Willuweit, Antje, Galldiks, Norbert ORCID: 0000-0002-2485-1796, Filss, Christian, Sabel, Michael C., Coenen, Heinz H., Shah, Nadim Jon and Langen, Karl-Josef ORCID: 0000-0003-1101-5075 (2019). Treatment-Related Uptake of O-(2-F-18-Fluoroethyl)-L-Tyrosine and L-[Methyl-H-3]-Methionine After Tumor Resection in Rat Glioma Models. J. Nucl. Med., 60 (10). S. 1373 - 1380. RESTON: SOC NUCLEAR MEDICINE INC. ISSN 1535-5667

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Abstract

Assessment of residual tumor after resection of cerebral gliomas can be difficult with MRI and may be improved by amino acid PET. The aim of this experimental study was to investigate uptake of 2-F-18-fluoroethyl-L-tyrosine (F-18-FET) and L-[methyl-H-3]-methionine (H-3-MET) in residual tumor after surgery and possible false-positive uptake in treatment-related changes. Methods: F98 or GS-9L rat gliomas were implanted into the brain of 64 rats. Tumors were resected after 1 wk of tumor growth, and sham surgery was performed in an additional 10 animals. At different time points after surgery (1, 2, 3, 7, and 14-16 d), rats underwent ex vivo dual-tracer autoradiography using F-18-FET and H-3-MET. Histologic slices were evaluated by immunostaining for cell density and astrogliosis. Tracer uptake was quantified by lesion-to-brain ratios (L/B) at the rim of the resection cavity (considered treatment-related uptake) and in residual or recurrent tumor tissue. Four animals showing no residual tumor underwent PET 3 d after surgery to examine time-activity curves of F-18-FET uptake in treatment-related changes. Results: Treatment-related uptake with a mean L/B of 2.0 +/- 0.3 for F-18-FET and a mean L/B of 1.7 +/- 0.2 for H-3-MET was noted at the rim of the resection cavity in the first week after surgery, decreasing significantly by 14-16 d (P < 0.01). Treatment-related tracer uptake was significantly higher for F-18-FET than for H-3-MET (P< 0.001). Tracer uptake in rat gliomas exceeded treatment-related tracer uptake at all time points (P < 0.001), but the latter was in the range of human gliomas. Reactive astrogliosis was noted near the resection cavity from the second day after surgery. Time-activity curves of F-18-FET uptake in those areas revealed constantly increasing uptake. Conclusion: Surgery may induce significant treatment-related F-18-FET and H-3-MET uptake near the resection cavity in the first week after surgery, presumably caused by reactive astrogliosis. Treatment-related tracer uptake was less pronounced for 3H-MET, indicating that C-11-MET may be better suited for assessing the postoperative situation than F-18-FET. Assessment of residual tumor after surgery by amino acid PET seems to be more reliable after an interval of 14 d.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Geisler, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Stegmayr, Carina UNSPECIFIED orcid.org/0000-0003-4191-8734 UNSPECIFIED Niemitz, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Lohmann, Philipp UNSPECIFIED orcid.org/0000-0002-5360-046X UNSPECIFIED Rapp, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Stoffels, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Willuweit, Antje UNSPECIFIED UNSPECIFIED UNSPECIFIED Galldiks, Norbert UNSPECIFIED orcid.org/0000-0002-2485-1796 UNSPECIFIED Filss, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Sabel, Michael C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Coenen, Heinz H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Shah, Nadim Jon UNSPECIFIED UNSPECIFIED UNSPECIFIED Langen, Karl-Josef UNSPECIFIED orcid.org/0000-0003-1101-5075 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-132873
DOI:	10.2967/jnumed.119.225680
Journal or Publication Title:	J. Nucl. Med.
Volume:	60
Number:	10
Page Range:	S. 1373 - 1380
Date:	2019
Publisher:	SOC NUCLEAR MEDICINE INC
Place of Publication:	RESTON
ISSN:	1535-5667
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POSITRON-EMISSION-TOMOGRAPHY; BRAIN-BARRIER PERMEABILITY; GLIOBLASTOMA-MULTIFORME; DIFFERENTIAL UPTAKE; F-18-FET PET; SURVIVAL; EXTENT; DIAGNOSIS; SURGERY; IMPACT Multiple languages Radiology, Nuclear Medicine & Medical Imaging Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13287