Universität zu Köln

Kaiser, Rainer W. J., Ignarski, Michael ORCID: 0000-0001-6057-7694, Van Nostrand, Eric L., Frese, Christian K., Jain, Manaswita, Cukoski, Sadrija, Heinen, Heide, Schaechter, Melanie, Seufert, Lisa, Bunte, Konstantin, Frommolt, Peter ORCID: 0000-0002-1966-8014, Keller, Patrick, Helm, Mark, Bohl, Katrin, Hoehne, Martin, Schermer, Bernhard, Benzing, Thomas, Hoepker, Katja, Dieterich, Christoph, Yeo, Gene W., Mueller, Roman-Ulrich and Fabretti, Francesca (2019). A protein-RNA interaction atlas of the ribosome biogenesis factor AATF. Sci Rep, 9. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

AATF is a central regulator of the cellular outcome upon p53 activation, a finding that has primarily been attributed to its function as a transcription factor. Recent data showed that AATF is essential for ribosome biogenesis and plays a role in rRNA maturation. AATF has been implicated to fulfil this role through direct interaction with rRNA and was identified in several RNA-interactome capture experiments. Here, we provide a first comprehensive analysis of the RNA bound by AATF using CLIP-sequencing. Interestingly, this approach shows predominant binding of the 45S pre-ribosomal RNA precursor molecules. Furthermore, AATF binds to mRNAs encoding for ribosome biogenesis factors as well as snoRNAs. These findings are complemented by an in-depth analysis of the protein interactome of AATF containing a large set of proteins known to play a role in rRNA maturation with an emphasis on the protein-RNA-complexes known to be required for the generation of the small ribosomal subunit (SSU). In line with this finding, the binding sites of AATF within the 45S rRNA precursor localize in close proximity to the SSU cleavage sites. Consequently, our multilayer analysis of the protein-RNA interactome of AATF reveals this protein to be an important hub for protein and RNA interactions involved in ribosome biogenesis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kaiser, Rainer W. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ignarski, Michael UNSPECIFIED orcid.org/0000-0001-6057-7694 UNSPECIFIED Van Nostrand, Eric L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Frese, Christian K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jain, Manaswita UNSPECIFIED UNSPECIFIED UNSPECIFIED Cukoski, Sadrija UNSPECIFIED UNSPECIFIED UNSPECIFIED Heinen, Heide UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaechter, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Seufert, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Bunte, Konstantin UNSPECIFIED UNSPECIFIED UNSPECIFIED Frommolt, Peter UNSPECIFIED orcid.org/0000-0002-1966-8014 UNSPECIFIED Keller, Patrick UNSPECIFIED UNSPECIFIED UNSPECIFIED Helm, Mark UNSPECIFIED UNSPECIFIED UNSPECIFIED Bohl, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoehne, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Schermer, Bernhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Benzing, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoepker, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Dieterich, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Yeo, Gene W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Roman-Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Fabretti, Francesca UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-134482
DOI:	10.1038/s41598-019-47552-3
Journal or Publication Title:	Sci Rep
Volume:	9
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language P53 TRANSCRIPTION; CELL-GROWTH; CHE-1; GENE; IDENTIFICATION; NUCLEOLUS; APOPTOSIS; DAMAGE; PHOSPHORYLATION; PROCESSOME Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13448