Universität zu Köln

Zhong, Nongping, Dong, Tao, Chen, Zhongchun, Guo, Yongwei ORCID: 0000-0001-9195-0770, Shao, Zhengzhong and Zhao, Xia ORCID: 0000-0002-2379-9447 (2019). A novel 3D-printed silk fibroin-based scaffold facilitates tracheal epithelium proliferation in vitro. J. Biomater. Appl., 34 (1). S. 3 - 12. LONDON: SAGE PUBLICATIONS LTD. ISSN 1530-8022

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Abstract

The functional epithelial regeneration is important for repairing tracheal defects. However, the potential of 3D-printed SF-based scaffolds for tracheal epithelial regeneration is still unknown. In this study, we developed a novel silk fibroin-based scaffold prepared by 3D printing of silk fibroin/hydroxypropyl methyl cellulose (SF/HPMC) thixotropic hydrogel and evaluated the tracheal epithelium proliferation on this scaffold in vitro. Combined with the freeze-dried technology, the 3D-printed SF/HPMC scaffolds had porous structures in the printed bars. After evaluation of their pore sizes, porosities, water contents and mechanical properties, the scaffolds were co-cultured with the normal human bronchial epithelial cell line (BEAS-2B) for seven days. We detected the BEAS-2B cells proliferation on the scaffolds using a CCK-8 assay, determined their mucin secretion and intercellular tight junction formation by immunofluorescence, as well as observed their cell viability and morphology by live/dead staining and scanning electronic microscopy. The results showed that the SF/HPMC scaffolds had good porosity, water content and mechanical properties. In addition, the BEAS-2B cells proliferated well on SF/HPMC scaffolds, during the seven-day co-culture, with high viability, mucin expression, and intercellular tight junction formation. In summary, these results demonstrated that the BEAS-2B cells could attach and proliferate on the 3D-printed SF/HPMC scaffolds, which were expected to have potential for facilitating tracheal epithelial regeneration.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zhong, Nongping UNSPECIFIED UNSPECIFIED UNSPECIFIED Dong, Tao UNSPECIFIED UNSPECIFIED UNSPECIFIED Chen, Zhongchun UNSPECIFIED UNSPECIFIED UNSPECIFIED Guo, Yongwei UNSPECIFIED orcid.org/0000-0001-9195-0770 UNSPECIFIED Shao, Zhengzhong UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhao, Xia UNSPECIFIED orcid.org/0000-0002-2379-9447 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-136783
DOI:	10.1177/0885328219845092
Journal or Publication Title:	J. Biomater. Appl.
Volume:	34
Number:	1
Page Range:	S. 3 - 12
Date:	2019
Publisher:	SAGE PUBLICATIONS LTD
Place of Publication:	LONDON
ISSN:	1530-8022
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language VIVO DEGRADATION; FABRICATION; RECONSTRUCTION; BIOMATERIALS; HYDROGELS; STENT Multiple languages Engineering, Biomedical; Materials Science, Biomaterials Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13678