Zhang, Bipei, Chopra, Divykriti, Schrader, Andrea ORCID: 0000-0002-3879-7057 and Huelskamp, Martin (2019). Evolutionary comparison of competitive protein-complex formation of MYB, bHLH, and WDR proteins in plants. J. Exp. Bot., 70 (12). S. 3197 - 3210. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2431

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Abstract

A protein complex consisting of a MYB, basic Helix-Loop-Helix, and a WDR protein, the MBW complex, regulates five traits, namely the production of anthocyanidin, proanthocyanidin, and seed-coat mucilage, and the development of trichomes and root hairs. For complexes involved in trichome and root hair development it has been shown that the interaction of two MBW proteins can be counteracted by the respective third protein (called competitive complex formation). We examined competitive complex formation for selected MBW proteins from Arabidopsis thaliana, Arabis alpina, Gossypium hirsutum, Petunia hybrida, and Zea mays. Quantitative analyses of the competitive binding of MYBs and WDRs to bHLHs were done by pull-down assays using ProtA- and luciferase-tagged proteins expressed in human HEC cells. We found that some bHLHs show competitive complex formation whilst others do not. Competitive complex formation strongly correlated with a phylogenetic tree constructed with the bHLH proteins under investigation, suggesting a functional relevance. We demonstrate that this different behavior can be explained by changes in one amino acid and that this position is functionally relevant in trichome development but not in anthocyanidin regulation.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Zhang, BipeiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chopra, DivykritiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schrader, AndreaUNSPECIFIEDorcid.org/0000-0002-3879-7057UNSPECIFIED
Huelskamp, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-138363
DOI: 10.1093/jxb/erz155
Journal or Publication Title: J. Exp. Bot.
Volume: 70
Number: 12
Page Range: S. 3197 - 3210
Date: 2019
Publisher: OXFORD UNIV PRESS
Place of Publication: OXFORD
ISSN: 1460-2431
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
HELIX-LOOP-HELIX; ARABIDOPSIS-THALIANA; TRICHOME DEVELOPMENT; ANTHOCYANIN PIGMENTATION; TRANSCRIPTION FACTORS; PETUNIA ENCODES; CELL FATE; GENES; TTG1; EXPRESSIONMultiple languages
Plant SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/13836

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