Junker, Niklas O., Vaghefikia, Farzaneh, Albarghash, Alyazan, Hoefig, Henning, Kempe, Daryan, Walter, Julia, Otten, Julia ORCID: 0000-0002-2015-1215, Pohl, Martina, Katranidis, Alexandros ORCID: 0000-0002-1785-1659, Wiegand, Simone ORCID: 0000-0001-6333-1956 and Fitter, Joerg ORCID: 0000-0002-4503-2079 (2019). Impact of Molecular Crowding on Translational Mobility and Conformational Properties of Biological Macromolecules. J. Phys. Chem. B, 123 (21). S. 4477 - 4487. WASHINGTON: AMER CHEMICAL SOC. ISSN 1520-6106

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Abstract

Effects of molecular crowding on structural and dynamical properties of biological macromolecules do depend on the concentration of crowding agents but also on the molecular mass and the structural compactness of the crowder molecules. By employing fluorescence correlation spectroscopy (FCS), we investigated the translational mobility of several biological macromolecules ranging from 17 kDa to 2.7 MDa. Polyethylene glycol and Ficoll polymers of different molecular masses were used in buffer solutions to mimic a crowded environment. The reduction in translational mobility of the biological tracer molecules was analyzed as a function of crowder volume fractions and was generally more pronounced in PEG as compared to Ficoll solutions. For several crowding conditions, we observed a molecular sieving effect, in which the diffusion coefficient of larger tracer molecules is reduced to a larger extent than predicted by the Stokes-Einstein relation. By employing a FRET-based biosensor, we also showed that a multiprotein complex is significantly compacted in the presence of macromolecular crowders. Importantly, with respect to sensor in vivo applications, ligand concentration determining sensors would need a crowding specific calibration in order to deliver correct cytosolic ligand concentration.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Junker, Niklas O.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vaghefikia, FarzanehUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Albarghash, AlyazanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoefig, HenningUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kempe, DaryanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Walter, JuliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Otten, JuliaUNSPECIFIEDorcid.org/0000-0002-2015-1215UNSPECIFIED
Pohl, MartinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Katranidis, AlexandrosUNSPECIFIEDorcid.org/0000-0002-1785-1659UNSPECIFIED
Wiegand, SimoneUNSPECIFIEDorcid.org/0000-0001-6333-1956UNSPECIFIED
Fitter, JoergUNSPECIFIEDorcid.org/0000-0002-4503-2079UNSPECIFIED
URN: urn:nbn:de:hbz:38-147733
DOI: 10.1021/acs.jpcb.9b01239
Journal or Publication Title: J. Phys. Chem. B
Volume: 123
Number: 21
Page Range: S. 4477 - 4487
Date: 2019
Publisher: AMER CHEMICAL SOC
Place of Publication: WASHINGTON
ISSN: 1520-6106
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ESCHERICHIA-COLI; PROTEIN; DIFFUSION; CONSEQUENCES; ENVIRONMENTS; ASSOCIATION; BIOSENSORS; CYTOPLASM; BINDINGMultiple languages
Chemistry, PhysicalMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/14773

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