Universität zu Köln

Papagiannouli, Fani ORCID: 0000-0003-4094-6688, Berry, Cameron Wynn and Fuller, Margaret T. (2019). The Dig Module and Clathrin-Mediated Endocytosis Regulate EGFR Signaling and Cyst Cell-Germline Coordination in the Drosophila Testis. Stem Cell Rep., 12 (5). S. 1024 - 1041. CAMBRIDGE: CELL PRESS. ISSN 2213-6711

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Abstract

Tissue homeostasis and repair relies on proper communication of stem cells and their differentiating daughters with the local tissue microenvironment. In the Drosophila male germline adult stem cell lineage, germ cells proliferate and progressively differentiate enclosed in supportive somatic cyst cells, forming a small organoid, the functional unit of differentiation. Here we show that cell polarity and vesicle trafficking influence signal transduction in cyst cells, with profound effects on the germ cells they enclose. Our data suggest that the cortical components Dlg, Scrib, Lgl and the clathrin-mediated endocytic (CME) machinery downregulate epidermal growth factor receptor (EGFR) signaling. Knockdown of dlg, scrib, lgl, or CME components in cyst cells resulted in germ cell death, similar to increased signal transduction via the EGFR, while lowering EGFR or downstream signaling components rescued the defects. This work provides insights into how cell polarity and endocytosis cooperate to regulate signal transduction and sculpt developing tissues.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Papagiannouli, Fani UNSPECIFIED orcid.org/0000-0003-4094-6688 UNSPECIFIED Berry, Cameron Wynn UNSPECIFIED UNSPECIFIED UNSPECIFIED Fuller, Margaret T. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-148262
DOI:	10.1016/j.stemcr.2019.03.008
Journal or Publication Title:	Stem Cell Rep.
Volume:	12
Number:	5
Page Range:	S. 1024 - 1041
Date:	2019
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2213-6711
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language STEM-CELLS; SELF-RENEWAL; DISCS LARGE; POLARITY; PROTEIN; EXPRESSION; HOMOLOG; PROLIFERATION; LOCALIZATION; TRAFFICKING Multiple languages Cell & Tissue Engineering; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14826