Hueynck, Jan Schulze, Kaschani, Farnusch, van der Linde, Karina ORCID: 0000-0003-1390-8344, Ziemann, Sebastian, Mueller, Andre N., Colby, Thomas, Kaiser, Markus, Villamil, Johana C. Misas and Doehlemann, Gunther (2019). Proteases Underground: Analysis of the Maize Root Apoplast Identifies Organ Specific Papain-Like Cysteine Protease Activity. Front. Plant Sci., 10. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-462X

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Abstract

Plant proteases are key regulators of plant cell processes such as seed development, immune responses, senescence and programmed cell death (PCD). Apoplastic papain-like cysteine proteases (PL) are hubs in plant-microbe interactions and play an important role during abiotic stresses. The apoplast is a crucial interface for the interaction between plant and microbes. So far, apoplastic maize PL and their function have been mostly described for aerial parts. In this study, we focused on apoplastic PLCPs in the roots of maize plants. We have analyzed the phylogeny of maize PLCPs and investigated their protein abundance after salicylic acid (SA) treatment. Using activity-based protein profiling (ABPP) we have identified a novel root-specific PLCP belonging to the RD21-like subfamily, as well as three SA activated PLCPs. The root specific PLCP CP1C shares sequence and structural similarities to known CP1-like proteases. Biochemical analysis of recombinant CP1C revealed different substrate specificities and inhibitor affinities compared to the related proteases. This study characterized a root-specific PLCP and identifies differences between the SA-dependent activation of PLCPs in roots and leaves.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Hueynck, Jan SchulzeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kaschani, FarnuschUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van der Linde, KarinaUNSPECIFIEDorcid.org/0000-0003-1390-8344UNSPECIFIED
Ziemann, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mueller, Andre N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Colby, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kaiser, MarkusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Villamil, Johana C. MisasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doehlemann, GuntherUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-150253
DOI: 10.3389/fpls.2019.00473
Journal or Publication Title: Front. Plant Sci.
Volume: 10
Date: 2019
Publisher: FRONTIERS MEDIA SA
Place of Publication: LAUSANNE
ISSN: 1664-462X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CATHEPSIN-B; PHYTOPHTHORA-INFESTANS; C1A CYSTEINE; GENE FAMILY; CELL-DEATH; ARABIDOPSIS; INHIBITORS; SENESCENCE; CYSTATIN; PROTEOMEMultiple languages
Plant SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/15025

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