Universität zu Köln

Safi, Sema, Frommholz, David, Reimann, Susanne, Goetz, Werner, Bourauel, Christoph, Neumann, Anna-Lena, Hoerauf, Achim, Ilges, Harald, Safi, Ali-Farid, Jaeger, Andreas, Huebner, Marc P. and Goelz, Lina (2019). Comparative study on serum-induced arthritis in the temporomandibular and limb joint of mice. Int. J. Rheum. Dis., 22 (4). S. 636 - 646. HOBOKEN: WILEY. ISSN 1756-185X

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Abstract

IntroductionThe subject of the present study was a systematic comparative analysis of the rheumatoid arthritis (RA)-induced pathomechanisms in the temporomandibular joint with those of the limb joints using the serum-induced arthritis K/BxN model. MethodsIn 18 BALB/c mice the induction of RA was performed according to the Kouskoff method. Another healthy cohort served as controls (n=12). Joint swelling of the paws was measured using a micrometer. Functional data were obtained analyzing locomotion. Three-dimensional examination of the temporomandibular joint was performed with micro-computed tomography imaging, followed by histological evaluation of the extremity joints and the temporomandibular joint. Additionally, immunohistochemical investigations were carried out to evaluate inflammatory and immunological changes. ResultsMeasurement of joint swelling showed a significant increase in the diameter of the paws, as well as a decrease in locomotor activity compared to control animals and the time before arthritis induction. Histological and immunohistochemical investigations showed clear signs of inflammation in the extremity joints. In contrast, no histological or immunohistochemical indications of an inflammatory process were detectable in the temporomandibular joint. In addition, the three-dimensional analysis by micro-computed tomography of the temporomandibular joints did not show any obvious morphological changes. ConclusionFor the first time, using the K/BxN model we could demonstrate that, due to its anatomical and mechanical conditions, the temporomandibular joint seems to be less susceptible to the initiation of RA compared to limb joints. Therefore, additional investigations are needed on other arthritis models as well, in order to further improve our understanding of the pathogenesis and defense mechanisms of the disease.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Safi, Sema UNSPECIFIED UNSPECIFIED UNSPECIFIED Frommholz, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Reimann, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Goetz, Werner UNSPECIFIED UNSPECIFIED UNSPECIFIED Bourauel, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Neumann, Anna-Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoerauf, Achim UNSPECIFIED UNSPECIFIED UNSPECIFIED Ilges, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Safi, Ali-Farid UNSPECIFIED UNSPECIFIED UNSPECIFIED Jaeger, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Huebner, Marc P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Goelz, Lina UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-151487
DOI:	10.1111/1756-185X.13486
Journal or Publication Title:	Int. J. Rheum. Dis.
Volume:	22
Number:	4
Page Range:	S. 636 - 646
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1756-185X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RHEUMATOID-ARTHRITIS; II COLLAGEN; OSTEOARTHRITIS; INFLAMMATION; INVOLVEMENT; CARTILAGE; ALPHA Multiple languages Rheumatology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15148