Universität zu Köln

Nizam, Shadab, Qiang, Xiaoyu, Wawra, Stephan ORCID: 0000-0001-8555-1618, Nostadt, Robin, Getzke, Felix, Schwanke, Florian, Dreyer, Ingo ORCID: 0000-0002-2781-0359, Langen, Gregor ORCID: 0000-0002-8321-1756 and Zuccaro, Alga ORCID: 0000-0002-8026-0114 (2019). Serendipita indica E5 ' NT modulates extracellular nucleotide levels in the plant apoplast and affects fungal colonization. EMBO Rep., 20 (2). HOBOKEN: WILEY. ISSN 1469-3178

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Abstract

Extracellular adenosine 5'-triphosphate (eATP) is an essential signaling molecule that mediates different cellular processes through its interaction with membrane-associated receptor proteins in animals and plants. eATP regulates plant growth, development, and responses to biotic and abiotic stresses. Its accumulation in the apoplast induces ROS production and cytoplasmic calcium increase mediating a defense response to invading microbes. We show here that perception of extracellular nucleotides, such as eATP, is important in plant-fungus interactions and that during colonization by the beneficial root endophyte Serendipita indica eATP accumulates in the apoplast at early symbiotic stages. Using liquid chromatography-tandem mass spectrometry, and cytological and functional analysis, we show that S. indica secrets SiE5'NT, an enzymatically active ecto-5'-nucleotidase capable of hydrolyzing nucleotides in the apoplast. Arabidopsis thaliana lines producing extracellular SiE5'NT are significantly better colonized, have reduced eATP levels, and altered responses to biotic stresses, indicating that SiE5'NT functions as a compatibility factor. Our data suggest that extracellular bioactive nucleotides and their perception play an important role in fungus-root interactions and that fungal-derived enzymes can modify apoplastic metabolites to promote fungal accommodation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nizam, Shadab UNSPECIFIED UNSPECIFIED UNSPECIFIED Qiang, Xiaoyu UNSPECIFIED UNSPECIFIED UNSPECIFIED Wawra, Stephan UNSPECIFIED orcid.org/0000-0001-8555-1618 UNSPECIFIED Nostadt, Robin UNSPECIFIED UNSPECIFIED UNSPECIFIED Getzke, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwanke, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Dreyer, Ingo UNSPECIFIED orcid.org/0000-0002-2781-0359 UNSPECIFIED Langen, Gregor UNSPECIFIED orcid.org/0000-0002-8321-1756 UNSPECIFIED Zuccaro, Alga UNSPECIFIED orcid.org/0000-0002-8026-0114 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-157748
DOI:	10.15252/embr.201847430
Journal or Publication Title:	EMBO Rep.
Volume:	20
Number:	2
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-3178
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AGROBACTERIUM-MEDIATED TRANSFORMATION; PIRIFORMOSPORA-INDICA; LACCARIA-BICOLOR; GENE-EXPRESSION; FLORAL DIP; PROTEIN; ARABIDOPSIS; ATP; CELL; EFFECTOR Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15774