Universität zu Köln

Seisenbayeva, A. S., Isachenko, V., Assanova, Y. A., Du, X. X., Toishybek, D. Y. and Toishibekov, Y. M. (2019). Optimization of medium for in vitro culture of sheep ovarian tissue. Small Ruminant Res., 171. S. 82 - 87. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1879-0941

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Abstract

Creation of genetic cryobanks is a way of preserving animal genetic materials. The gene bank can be used actively for increasing populations as well as minimization of interrelated crossing. One of the ways of preserving animal genetic material is ovarian tissue cryopreservation. After cryopreservation and storage, part of the ovarian tissue can be thawed and cultured in vitro in order to check a quality of follicles and stromal cells. The viability rate indicates whether or not it is possible to restore the reproductive function of the animal. Our study aimed to search and test a new medium for in vitro culture of sheep ovarian tissue. Ovarian pieces from 32 ewes (n = 320 and n = 180 in two series of experiments) were cultured during 16 days in three basic media: Tissue Culture Medium (TCM) 199, Buffered Tissue Culture Medium (TCM-HEPES), and Dulbecco Modified Eagle Medium (DMEM). These basic culture media used in different experimental groups (12 and 6) were supplemented with fetal calf serum (FCS), ewe estrous serum (EES) as well as follicles stimulating hormone and luteinizing hormone (FSH and LH, respectively). After the cultures of ovarian tissue and histological treatment, respectively, developmental rates of primordial, primary and secondary follicles as well as the secretion of hormones by tissues (progesterone and estradiol 17 beta) were evaluated. Maximal developmental rate of follicles and hormonal activity were observed in the group with the culture medium TCM-HEPES + EES + FSH + LH. For in vitro culture of sheep ovarian tissue, the following medium is recommended: TCM-HEPES (supplemented with 100 mu g/mL penicillin-streptomycin, 50 mu g/mL gentamicin, and 2 mM L-Glutamine) + 10% EES + 5 mu g/mL FSH + 5 mu g/mL LH.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Seisenbayeva, A. S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Assanova, Y. A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Du, X. X. UNSPECIFIED UNSPECIFIED UNSPECIFIED Toishybek, D. Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Toishibekov, Y. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-157964
DOI:	10.1016/j.smallrumres.2018.10.011
Journal or Publication Title:	Small Ruminant Res.
Volume:	171
Page Range:	S. 82 - 87
Date:	2019
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1879-0941
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PRIMORDIAL FOLLICLES; OOCYTE MATURATION; CORTICAL TISSUE; CRYOPRESERVATION; BIOTECHNOLOGIES; GROWTH; SERUM Multiple languages Agriculture, Dairy & Animal Science Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15796