Universität zu Köln

Wagner, I., V, Yango, P., Svechnikov, K., Tran, N. D. and Soder, O. (2019). Adipocytokines may delay pubertal maturation of human Sertoli cells. Reprod. Fertil. Dev., 31 (8). S. 1395 - 1401. CLAYTON: CSIRO PUBLISHING. ISSN 1448-5990

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Abstract

Reproduction is an important target of obesity complications, including adverse effects on spermatogenesis and steroidogenesis. Adipocytokines are key mediators in various complications of obesity. Our aim was to study the potential of adipocytokines to affect Sertoli cell function, which is crucial for spermatogenesis, and possibly link these findings to the observed attenuation of spermatogenesis in obese males. Testicular biopsies were obtained from healthy donors. Highly purified adult human Sertoli cells (HSCs) were isolated by fluorescence-activated cell sorting. Cells were cultured and exposed to different concentrations of adipocytokines (10-1000ngmL(-1)) for 2-7 days. Expression of selected Sertoli cell genes was quantified by quantitative polymerase chain reaction. Long-term treatment (7 days) of HSCs with higher concentrations of chemerin, irisin, nicotinamide phosphoribosyltransferase (Nampt), resistin and progranulin significantly suppressed FSH receptor expression (by 79%, 83%, 64%, 71% and 26% respectively; P<0.005 for all) and significantly upregulated cytochrome P450 family 26 subfamily A member 1 (CYP26A1) expression (by 48%, 90%, 126%, 126% and 153% respectively P<0.005 for all), comparable to what is found in the prepubertal state. Further, these adipocytokines significantly attenuated the expression of bone morphogenetic protein-4, glial cell line-derived neurotrophic factor, leukaemia inhibitory factor and fibroblast growth factor-2 by HSCs. We propose that adipocytokines, at high concentrations, which are often observed in obese males when tested invitro, may negatively affect Sertoli cell maturation and retain these cells in a more prepubertal stage. This could negatively affect testis function and add to fertility problems in obese adults.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wagner, I., V UNSPECIFIED UNSPECIFIED UNSPECIFIED Yango, P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Svechnikov, K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tran, N. D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Soder, O. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-160857
DOI:	10.1071/RD18487
Journal or Publication Title:	Reprod. Fertil. Dev.
Volume:	31
Number:	8
Page Range:	S. 1395 - 1401
Date:	2019
Publisher:	CSIRO PUBLISHING
Place of Publication:	CLAYTON
ISSN:	1448-5990
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TESTICULAR STEROIDOGENESIS; OBESITY; EXPRESSION; ADIPONECTIN; LEPTIN; TESTIS; TESTOSTERONE; ADIPOKINES; DISORDERS; RECEPTORS Multiple languages Developmental Biology; Reproductive Biology; Zoology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16085