Universität zu Köln

Delporte, Marianne ORCID: 0000-0003-4340-2440, Bernard, Guillaume, Legrand, Guillaume, Hielscher, Bjoern, Lanoue, Arnaud, Molinie, Roland, Rambaud, Caroline, Mathiron, David, Besseau, Sebastien, Linka, Nicole, Hilbert, Jean-Louis and Gagneul, David (2018). A BAHD neofunctionalization promotes tetrahydroxycinnamoyl spermine accumulation in the pollen coat of the Asteraceae family. J. Exp. Bot., 69 (22). S. 5355 - 5372. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2431

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Abstract

In eudicotyledons, accumulation of trihydroxycinnamoyl spermidine that is restricted to the pollen wall constitutes an evolutionary conserved trait. However, the role of this compound, which is synthetized by the BAHD enzyme spermidine hydroxycinnamoyl transferase (SHT), is still a matter of debate. Here, we show that this particular phenolamide is replaced by tetrahydroxycinnamoyl spermine in the pollen coat of the Asteraceae. Phylogenetic analyses combined with quantitative RT-PCR experiments allowed the identification of two homologous genes from Cichorium intybus (chicory) putatively involved in its metabolism. In vitro biochemical characterization of the two enzymes, named CiSHT1 and CiSHT2, confirmed the capability of recombinant proteins to synthesize spermine as well as spermidine derivatives. The wild-type metabolic phenotype was partially restored in an Arabidopsis sht mutant expressing CiSHT2. Strikingly, the transgenic plants also accumulated spermine derivatives that were absent in the wild-type. Overexpression of CiSHT2 in chicory hairy roots led to the accumulation of spermine derivatives, confirming its in vivo function. Complementary sequence analyses revealed the presence of an amino acid motif typical of the SHTs among the BAHD enzyme family. Our results highlight a recent neofunctionalization among the SHTs that has promoted the emergence of new phenolamides in the Asteraceae, which could potentially have contributed to the evolutionary success of this family.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Delporte, Marianne UNSPECIFIED orcid.org/0000-0003-4340-2440 UNSPECIFIED Bernard, Guillaume UNSPECIFIED UNSPECIFIED UNSPECIFIED Legrand, Guillaume UNSPECIFIED UNSPECIFIED UNSPECIFIED Hielscher, Bjoern UNSPECIFIED UNSPECIFIED UNSPECIFIED Lanoue, Arnaud UNSPECIFIED UNSPECIFIED UNSPECIFIED Molinie, Roland UNSPECIFIED UNSPECIFIED UNSPECIFIED Rambaud, Caroline UNSPECIFIED UNSPECIFIED UNSPECIFIED Mathiron, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Besseau, Sebastien UNSPECIFIED UNSPECIFIED UNSPECIFIED Linka, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Hilbert, Jean-Louis UNSPECIFIED UNSPECIFIED UNSPECIFIED Gagneul, David UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-162788
DOI:	10.1093/jxb/ery320
Journal or Publication Title:	J. Exp. Bot.
Volume:	69
Number:	22
Page Range:	S. 5355 - 5372
Date:	2018
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2431
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HYDROXYCINNAMOYL-COENZYME; SPECIALIZED METABOLISM; ARABIDOPSIS-THALIANA; ACYLTRANSFERASES; BIOSYNTHESIS; EXPRESSION; POLYAMINES; IDENTIFICATION; TRANSFERASES; SPERMIDINES Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16278