Kopp, Sascha, Krueger, Marcus, Bauer, Johann, Wehland, Markus ORCID: 0000-0002-8160-859X, Corydon, Thomas J., Sahana, Jayashree ORCID: 0000-0002-6606-6315, Nassef, Mohamed Zakaria, Melnik, Daniela, Bauer, Thomas J., Schulz, Herbert, Schuette, Andreas, Schmitz, Burkhard, Oltmann, Hergen, Feldmann, Stefan, Infanger, Manfred and Grimm, Daniela (2018). Microgravity Affects Thyroid Cancer Cells during the TEXUS-53 Mission Stronger than Hypergravity. Int. J. Mol. Sci., 19 (12). BASEL: MDPI. ISSN 1422-0067

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Abstract

Thyroid cancer is the most abundant tumor of the endocrine organs. Poorly differentiated thyroid cancer is still difficult to treat. Human cells exposed to long-term real (r-) and simulated (s-) microgravity (mu g) revealed morphological alterations and changes in the expression profile of genes involved in several biological processes. The objective of this study was to examine the effects of short-term mu g on poorly differentiated follicular thyroid cancer cells (FTC-133 cell line) resulting from 6 min of exposure to mu g on a sounding rocket flight. As sounding rocket flights consist of several flight phases with different acceleration forces, rigorous control experiments are mandatory. Hypergravity (hyper-g) experiments were performed at 18g on a centrifuge in simulation of the rocket launch and s-mu g was simulated by a random positioning machine (RPM). qPCR analyses of selected genes revealed no remarkable expression changes in controls as well as in hyper-g samples taken at the end of the first minute of launch. Using a centrifuge initiating 18g for 1 min, however, presented moderate gene expression changes, which were significant for COL1A1, VCL, CFL1, PTK2, IL6,CXCL8 and MMP14. We also identified a network of mutual interactions of the investigated genes and proteins by employing in-silico analyses. Lastly, mu g-samples indicated that microgravity is a stronger regulator of gene expression than hyper-g.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kopp, SaschaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krueger, MarcusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bauer, JohannUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wehland, MarkusUNSPECIFIEDorcid.org/0000-0002-8160-859XUNSPECIFIED
Corydon, Thomas J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sahana, JayashreeUNSPECIFIEDorcid.org/0000-0002-6606-6315UNSPECIFIED
Nassef, Mohamed ZakariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Melnik, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bauer, Thomas J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schulz, HerbertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schuette, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schmitz, BurkhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Oltmann, HergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Feldmann, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Infanger, ManfredUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grimm, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-162921
DOI: 10.3390/ijms19124001
Journal or Publication Title: Int. J. Mol. Sci.
Volume: 19
Number: 12
Date: 2018
Publisher: MDPI
Place of Publication: BASEL
ISSN: 1422-0067
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SIMULATED MICROGRAVITY; EXTRACELLULAR-MATRIX; 3-DIMENSIONAL GROWTH; ERM PROTEINS; CYTOSKELETON; ADHESION; GRAVITY; BINDING; MOESIN; TALINMultiple languages
Biochemistry & Molecular Biology; Chemistry, MultidisciplinaryMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/16292

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