Okmen, Bilal, Mathow, Daniel, Hof, Alexander, Lahrmann, Urs, Assmann, Daniela and Doehlemann, Gunther (2018). Mining the effector repertoire of the biotrophic fungal pathogen Ustilago hordei during host and non-host infection. Mol. Plant Pathol., 19 (12). S. 2603 - 2623. HOBOKEN: WILEY. ISSN 1364-3703

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Abstract

The success of plant-pathogenic fungi mostly relies on their arsenal of virulence factors which are expressed and delivered into the host tissue during colonization. The biotrophic fungal pathogen Ustilago hordei causes covered smut disease on both barley and oat. In this study, we combined cytological, genomics and molecular biological methods to achieve a better understanding of the molecular interactions in the U. hordei-barley pathosystem. Microscopic analysis revealed that U. hordei densely colonizes barley leaves on penetration, in particular the vascular system. Transcriptome analysis of U. hordei at different stages of host infection revealed differential expression of the transcript levels of 273 effector gene candidates. Furthermore, U. hordei transcriptionally activates core effector genes which may suppress even non-host early defence responses. Based on expression profiles and novelty of sequences, knockout studies of 14 effector candidates were performed in U. hordei, which resulted in the identification of four virulence factors required for host colonization. Yeast two-hybrid screening identified potential barley targets for two of the effectors. Overall, this study provides a first systematic analysis of the effector repertoire of U. hordei and identifies four effectors (Uvi1-Uvi4) as virulence factors for the infection of barley.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Okmen, BilalUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mathow, DanielUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hof, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lahrmann, UrsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Assmann, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doehlemann, GuntherUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-164101
DOI: 10.1111/mpp.12732
Journal or Publication Title: Mol. Plant Pathol.
Volume: 19
Number: 12
Page Range: S. 2603 - 2623
Date: 2018
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1364-3703
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CHITIN-TRIGGERED IMMUNITY; CELL-DEATH SUPPRESSOR; PHYTOPHTHORA-INFESTANS; DISEASE RESISTANCE; MAGNAPORTHE-ORYZAE; BAX INHIBITOR-1; PROTEASE INHIBITOR; ANTIFUNGAL PROTEIN; SMUT FUNGUS; MAYDISMultiple languages
Plant SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/16410

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