Gebauer, Jan M., Koehler, Anna, Dietmar, Helen, Gompert, Monika, Neundorf, Ines ORCID: 0000-0001-6450-3991, Zaucke, Frank ORCID: 0000-0002-7680-9354, Koch, Manuel and Baumann, Ulrich (2018). COMP and TSP-4 interact specifically with the novel GXKGHR motif only found in fibrillar collagens. Sci Rep, 8. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

Full text not available from this repository.

Abstract

COMP (cartilage oligomeric matrix protein) is a member of the thrombospondin family and forms homopentamers as well as mixed heterooligomers with its closely related family member TSP-4. COMP is long known to bind to collagens and to influence collagen fibril formation. Recent work indicates that already intracellular interaction with collagen is important for collagen secretion. However, the exact binding site of COMP on the collagen triple helix has not been described up to now. In this study we have identified a GXKGHR motif on the collagen II helix to bind to COMP, using a recombinantly expressed collagen II peptide library. This binding sequence is conserved throughout evolution and we demonstrate that TSP-4 binds to the same sequence. The identified binding motif overlaps with the recognition sites of many other collagen-binding partners (e.g. PEDF, Heparin) and also spans the lysine residues, which form collagen cross-links. COMP might thereby protect collagen helices from premature modification and cross-linking. Interestingly, this motif is only found in classical fibrillar collagens, although COMP is known to also bind other types. This might indicate that COMP has a unique interface for fibrillar collagens, thus making it an interesting target for the development of antifibrotic drugs.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Gebauer, Jan M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koehler, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dietmar, HelenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gompert, MonikaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Neundorf, InesUNSPECIFIEDorcid.org/0000-0001-6450-3991UNSPECIFIED
Zaucke, FrankUNSPECIFIEDorcid.org/0000-0002-7680-9354UNSPECIFIED
Koch, ManuelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Baumann, UlrichUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-165500
DOI: 10.1038/s41598-018-35447-8
Journal or Publication Title: Sci Rep
Volume: 8
Date: 2018
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2045-2322
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
OLIGOMERIC MATRIX PROTEIN; CRYSTAL-STRUCTURE; SIGNATURE DOMAIN; I COLLAGEN; CARTILAGE; BINDING; THROMBOSPONDIN-4; CALCIUM; PARTNERS; HSP47Multiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/16550

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item