Oekmen, Bilal, Kemmerich, Bastian, Hilbig, Daniel, Wemhoener, Raphael, Aschenbroich, Joern, Perrar, Andreas ORCID: 0000-0003-3365-5575, Huesgen, Pitter F., Schipper, Kerstin and Doehlemann, Gunther (2018). Dual function of a secreted fungalysin metalloprotease in Ustilago maydis. New Phytol., 220 (1). S. 249 - 262. HOBOKEN: WILEY. ISSN 1469-8137

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Abstract

Fungalysins from several phytopathogenic fungi have been shown to be involved in cleavage of plant chitinases. While fungal chitinases are responsible for cell wall remodeling during growth and morphogenesis, plant chitinases are important components of immunity. This study describes a dual function of the Ustilago maydis fungalysin UmFly1 in modulation of both plant and fungal chitinases. Genetic, biochemical and microscopic experiments were performed to elucidate the in vitro and in planta functions of U.maydis UmFly1. U.maydis Delta umfly1 mutants show significantly reduced virulence, which coincides with reduced cleavage of the maize chitinase ZmChiA within its chitin-binding domain. Moreover, deletion of umfly1 affected the cell separation of haploid U.maydis sporidia. This phenotype is associated with posttranslational activation of the endogenous chitinase UmCts1. Genetic complementation of the Delta umfly1 mutant with a homologous gene from closely related, but nonpathogenic, yeast fully rescued the cell separation defect invitro, but it could not recover the Delta umfly1 defect in virulence and cleavage of the maize chitinase. We report on the dual function of the secreted fungalysin UmFly1. We hypothesize that co-evolution of U.maydis with its host plant extended the endogenous function of UmFly1 towards the modulation of plant chitinase activity to promote infection.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Oekmen, BilalUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kemmerich, BastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hilbig, DanielUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wemhoener, RaphaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Aschenbroich, JoernUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Perrar, AndreasUNSPECIFIEDorcid.org/0000-0003-3365-5575UNSPECIFIED
Huesgen, Pitter F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schipper, KerstinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doehlemann, GuntherUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-172266
DOI: 10.1111/nph.15265
Journal or Publication Title: New Phytol.
Volume: 220
Number: 1
Page Range: S. 249 - 262
Date: 2018
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1469-8137
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CHITIN-TRIGGERED IMMUNITY; PLANT CHITINASES; VIRULENCE FACTOR; CELL-SEPARATION; EFFECTOR ECP6; PROTEIN; FUNGI; WHEAT; REVEALS; GROWTHMultiple languages
Plant SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/17226

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