Tateng, Aime Ngouateu, Kirstein, Oscar David, Ngouateu, Omer Bebe, Krueger, Andreas, von Stebut, Esther, Maurer, Marcus, Payne, Vincent Khan, Warburg, Alon and Dondji, Blaise (2018). First detection of Leishmania donovani in sand flies from Cameroon and its epidemiological implications. Trop. Med. Int. Health, 23 (9). S. 1014 - 1022. HOBOKEN: WILEY. ISSN 1365-3156
Full text not available from this repository.Abstract
ObjectivesA sound knowledge of the vector-host-parasite transmission dynamics is a prerequisite for adequate control measures of vector-borne diseases. To achieve this, an entomological investigation was conducted in the cutaneous leishmaniasis (CL) endemic focus of Mokolo District, northern Cameroon to identify the insect vector(s) of the disease. MethodsPhlebotomine sand flies were collected in and around Mokolo using New Standard CDC Miniature Light Traps. Individual sand flies were used for morphological species identification, and the remainder of the body for DNA analysis. Sand flies were demonstrated to harbour Leishmania spp. parasites using ITS1 PCR. Mitochondrial vertebrate-specific Cytochrome b -PCR was used to identify blood meals ingested by female sand flies. PCR amplicons were sequenced for Leishmania and blood sources discrimination. ResultsThis study revealed the presence of Leishmania donovani complex DNA (n = 1) in Phlebotomus duboscqi and of lizard-borne Leishmania tarentolae-like DNA (n = 3) in Sergentomyia spp. in 79 sand fly specimens from Mokolo district. ConclusionsThe causative agent of CL could not be detected in potential vectors. Instead, we found evidence for visceral leishmaniasis (VL) parasites in Phlebotomus duboscqi as well as enzootic reptile parasites in the Mokolo area. We recommend that an epidemiological survey be carried out in the area to evaluate the prevalence and eventually describe the clinical manifestations of VL in the human population. Political instability in neighbouring countries and the resulting refugee migration are likely explanations for the emergence of VL in Mokolo. ObjectifsUne bonne connaissance de la dynamique de transmission vecteur-hote-parasite est une condition prealable a des mesures de lutte adequates contre les maladies a transmission vectorielle. Pour ce faire, une etude entomologique a ete menee dans le foyer endemique de la leishmaniose cutanee (LC) du district de Mokolo, dans le nord du Cameroun, afin d'identifier le ou les insectes vecteurs de la maladie. MethodesDes phlebotomes ont ete collectes dans et autour de Mokolo a l'aide de nouveaux pieges standard a lumiere miniature du CDC. Des phlebotomes individuels ont ete utilises pour l'identification morphologique des especes et le reste du corps pour l'analyse de l'ADN. Il a ete demontre que les phlebotomes abritaient des parasites Leishmania spp. en utilisant la ITS1 PCR. La PCR basee sur le cytochrome b mitochondrial specifique aux vertebres a ete utilisee pour identifier les repas de sang ingeres par les phlebotomes femelles. Les amplicons PCR ont ete sequences pour distinguer entre Leishmania et les sources sanguines. ResultatsCette etude a revele la presence de l'ADN du complexe Leishmania donovani (n=1) chez Phlebotomus duboscqi et de l'ADN de type Leishmania tarentolae porte par des lezards (n=3) chez Sergentomyia spp., dans 79 specimens de mouches de sable du district de Mokolo. ConclusionsL'agent causal de la LC n'a pas pu etre detecte chez les vecteurs potentiels. Par contre, nous avons trouve des preuves de parasites de la leishmaniose viscerale (LV) dans Phlebotomus duboscqi ainsi que dans des parasites de reptiles enzootiques dans la region de Mokolo. Nous recommandons qu'une enquete epidemiologique soit menee dans la region pour evaluer la prevalence et eventuellement decrire les manifestations cliniques de la LV dans la population humaine. L'instabilite politique dans les pays voisins et la migration des refugies qui en resulte sont des explications probables de l'emergence de la LV a Mokolo.
| Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||||||
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| URN: | urn:nbn:de:hbz:38-173985 | ||||||||||||||||||||||||||||||||||||||||
| DOI: | 10.1111/tmi.13123 | ||||||||||||||||||||||||||||||||||||||||
| Journal or Publication Title: | Trop. Med. Int. Health | ||||||||||||||||||||||||||||||||||||||||
| Volume: | 23 | ||||||||||||||||||||||||||||||||||||||||
| Number: | 9 | ||||||||||||||||||||||||||||||||||||||||
| Page Range: | S. 1014 - 1022 | ||||||||||||||||||||||||||||||||||||||||
| Date: | 2018 | ||||||||||||||||||||||||||||||||||||||||
| Publisher: | WILEY | ||||||||||||||||||||||||||||||||||||||||
| Place of Publication: | HOBOKEN | ||||||||||||||||||||||||||||||||||||||||
| ISSN: | 1365-3156 | ||||||||||||||||||||||||||||||||||||||||
| Language: | English | ||||||||||||||||||||||||||||||||||||||||
| Faculty: | Unspecified | ||||||||||||||||||||||||||||||||||||||||
| Divisions: | Unspecified | ||||||||||||||||||||||||||||||||||||||||
| Subjects: | no entry | ||||||||||||||||||||||||||||||||||||||||
| Uncontrolled Keywords: |
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| Refereed: | Yes | ||||||||||||||||||||||||||||||||||||||||
| URI: | http://kups.ub.uni-koeln.de/id/eprint/17398 |
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