Universität zu Köln

Zuelbahar, Selen, Sieglitz, Florian, Kottmeier, Rita, Altenhein, Benjamin, Rumpf, Sebastian and Klaembt, Christian (2018). Differential expression of Obek controls ploidy in the Drosophila blood-brain barrier. Development, 145 (15). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9129

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Abstract

During development, tissue growth is mediated by either cell proliferation or cell growth, coupled with polyploidy. Both strategies are employed by the cell types that make up the Drosophila blood-brain barrier. During larval growth, the perineurial glia proliferate, whereas the subperineurial glia expand enormously and become polyploid. Here, we show that the level of ploidy in the subperineurial glia is controlled by the N-terminal asparagine amidohydrolase homolog Obek, and high Obek levels are required to limit replication. In contrast, perineurial glia express moderate levels of Obek, and increased Obek expression blocks their proliferation. Interestingly, other dividing cells are not affected by alteration of Obek expression. In glia, Obek counteracts fibroblast growth factor and Hippo signaling to differentially affect cell growth and number. We propose a mechanism by which growth signals are integrated differentially in a glia-specific manner through different levels of Obek protein to adjust cell proliferation versus endoreplication in the blood-brain barrier.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zuelbahar, Selen UNSPECIFIED UNSPECIFIED UNSPECIFIED Sieglitz, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Kottmeier, Rita UNSPECIFIED UNSPECIFIED UNSPECIFIED Altenhein, Benjamin UNSPECIFIED UNSPECIFIED UNSPECIFIED Rumpf, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Klaembt, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-178087
DOI:	10.1242/dev.164111
Journal or Publication Title:	Development
Volume:	145
Number:	15
Date:	2018
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9129
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language N-END RULE; VALOSIN-CONTAINING-PROTEIN; GLIAL-CELLS; EMERGING FUNCTIONS; MIGRATION; UBIQUITIN; ORGANIZATION; DEGRADATION; PATHWAY; PROLIFERATION Multiple languages Developmental Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17808